Reference : Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-sp...

	Document type :	E-prints/Working papers : Already available on another site
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	Focus Areas :	Systems Biomedicine
	To cite this reference:	http://hdl.handle.net/10993/39342

Title :	Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids
Language :	English
Author, co-author :	Smits, Lisa [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)]
	Magni, Stefano [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Grzyb, Kamil [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Antony, Paul [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Krüger, Rejko [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
	Skupin, Alexander [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Bolognin, Silvia [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Schwamborn, Jens Christian [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Publication date :	28-Mar-2019
Peer reviewed :	No
Abstract :	[en] Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organisation and function of a respective organ or part of an organ. Human midbrain organoids (hMOs) have been described to contain midbrain-specific dopaminergic neurons that release the neurotransmitter dopamine. However, the human midbrain contains also additional neuronal cell types, which are functionally interacting with each other. Here, we analysed hMOs at high-resolution by means of single-cell RNA-sequencing (scRNA-seq), imaging and electrophysiology to unravel cell heterogeneity. Our findings demonstrate that hMOs show essential neuronal functional properties as spontaneous electrophysiological activity of different neuronal subtypes, including dopaminergic, GABAergic, and glutamatergic neurons. Recapitulating these in vivo features makes hMOs an excellent tool for in vitro disease phenotyping and drug discovery.
Permalink :	http://hdl.handle.net/10993/39342
DOI :	10.1101/589598
source URL :	https://www.biorxiv.org/content/10.1101/589598v1

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