Reference : Generation of two induced pluripotent stem cell lines and the corresponding isogenic ...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/10993/54802

Title :	Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.1290A > G (p.T351A) or c.2067A > G (p.T610A) in the RHOT1 gene encoding Miro1.
Language :	English
Author, co-author :	Chemla, Axel [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
	Arena, Giuseppe [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
	Saraiva, Claudia [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Developmental and Cellular Biology]
	Berenguer-Escuder, Clara [> >]
	Grossmann, Dajana [> >]
	Grünewald, Anne [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Molecular and Functional Neurobiology]
	Klein, Christine [> >]
	Seibler, Philip [> >]
	Schwamborn, Jens Christian [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Developmental and Cellular Biology]
	Krüger, Rejko [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
Publication date :	2023
Journal title :	Stem cell research
Volume :	69
Pages :	103085
Peer reviewed :	Yes
ISSN :	1873-5061
e-ISSN :	1876-7753
Country :	England
Abstract :	[en] Primary skin fibroblasts from two Parkinson's disease (PD) patients carrying distinct heterozygous mutations in the RHOT1 gene encoding Miro1, namely c.1290A > G (Miro1 p.T351A) and c.2067A > G (Miro1 p.T610A), were converted into induced pluripotent stem cells (iPSCs) by episomal reprogramming. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. Here, we provide a comprehensive characterization and quality assurance of both isogenic pairs, which will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).
Permalink :	http://hdl.handle.net/10993/54802
Commentary :	Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
FnR project :	FnR ; FNR11676395 > Rejko Krüger > MiRisk-PD > Mitochondrial Risk Factors In Parkinson's Disease > 01/03/2018 > 31/08/2021 > 2017

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