Reference : Alterations of oral microbiota and impact on the gut microbiome in type 1 diabetes me...
Scientific journals : Article
Life sciences : Microbiology
Systems Biomedicine
http://hdl.handle.net/10993/54243
Alterations of oral microbiota and impact on the gut microbiome in type 1 diabetes mellitus revealed by integrated multi-omic analyses
English
Kunath, Benoît mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
Hickl, Oskar mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Bioinformatics Core >]
Teixeira Queiros, Pedro mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
Martin-Gallausiaux, Camille mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
Lebrun, Laura mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
Halder, Rashi mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Scientific Central Services >]
Laczny, Cedric Christian mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
Schmidt, Thomas Sebastian mailto [European Molecular Biology Laboratory, Heidelberg, Germany > Structural and Computational Biology Unit]
Hayward, Matthew mailto [European Molecular Biology Laboratory, Heidelberg, Germany > Structural and Computational Biology Unit]
Becher, Dorte mailto [Institute of Microbiology, University of Greifswald, Greifswald, Germany]
Heintz-Buschart, Anna mailto [Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands]
de Beaufort, Carine mailto [University of Luxembourg > > >]
Bork, Peer mailto [European Molecular Biology Laboratory, Heidelberg, Germany > Structural and Computational Biology Unit,]
May, Patrick mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Bioinformatics Core >]
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
Dec-2022
Microbiome
BioMed Central
Yes
International
2049-2618
London
United Kingdom
[en] Microbiome ; Type 1 Diabetes ; Meta-Omics
[en] Background: Alterations to the gut microbiome have been linked to multiple chronic diseases. However, the drivers of such changes remain largely unknown. The oral cavity acts as a major route of exposure to exogenous factors including pathogens, and processes therein may affect the communities in the subsequent compartments of the gastrointestinal tract. Here, we perform strain‑resolved, integrated meta‑genomic, transcriptomic, and proteomic analyses of paired saliva and stool samples collected from 35 individuals from eight families with multiple cases of type 1 diabetes mellitus (T1DM).
Results: We identified distinct oral microbiota mostly reflecting competition between streptococcal species. More specifically, we found a decreased abundance of the commensal Streptococcus salivarius in the oral cavity of T1DM individuals, which is linked to its apparent competition with the pathobiont Streptococcus mutans. The decrease in S. salivarius in the oral cavity was also associated with its decrease in the gut as well as higher abundances in facultative anaerobes including Enterobacteria. In addition, we found evidence of gut inflammation in T1DM as reflected in the expression profiles of the Enterobacteria as well as in the human gut proteome. Finally, we were able to follow transmitted strain‑variants from the oral cavity to the gut at the individual omic levels, highlighting not only the transfer, but also the activity of the transmitted taxa along the gastrointestinal tract.
Conclusions: Alterations of the oral microbiome in the context of T1DM impact the microbial communities in the lower gut, in particular through the reduction of “mouth‑to‑gut” transfer of Streptococcus salivarius. Our results indicate that the observed oral‑cavity‑driven gut microbiome changes may contribute towards the inflammatory processes involved in T1DM. Through the integration of multi‑omic analyses, we resolve strain‑variant “mouth‑to‑gut” transfer in a disease context.
Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group) ; Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (R. Schneider Group)
Fonds National de la Recherche - FnR
Researchers
http://hdl.handle.net/10993/54243
10.1186/s40168-022-01435-4
https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-022-01435-4
FnR ; FNR10404093 > Paul Wilmes > microCancer > Non-invasive Microbiome-derived Multi-omic Biomarkers For Early-stage Colorectal Cancer Detection > 01/01/2016 > 30/04/2019 > 2015

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