Alterations of oral microbiota and impact on the gut microbiome in type 1 diabetes mellitus revealed by integrated multi-omic analyses

Kunath, Benoît; Hickl, Oskar; Teixeira Queiros, Pedro; Martin-Gallausiaux, Camille; Lebrun, Laura; Halder, Rashi; Laczny, Cedric Christian; Schmidt, Thomas Sebastian; Hayward, Matthew; Becher, Dorte; Heintz-Buschart, Anna; de Beaufort, Carine; Bork, Peer; May, Patrick; Wilmes, Paul

doi:10.1186/s40168-022-01435-4

Reference : Alterations of oral microbiota and impact on the gut microbiome in type 1 diabetes me...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology
	Focus Areas :	Systems Biomedicine
	To cite this reference:	http://hdl.handle.net/10993/54243

Title :	Alterations of oral microbiota and impact on the gut microbiome in type 1 diabetes mellitus revealed by integrated multi-omic analyses
Language :	English
Author, co-author :	Kunath, Benoît [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
	Hickl, Oskar [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Bioinformatics Core >]
	Teixeira Queiros, Pedro [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
	Martin-Gallausiaux, Camille [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
	Lebrun, Laura [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
	Halder, Rashi [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Scientific Central Services >]
	Laczny, Cedric Christian [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
	Schmidt, Thomas Sebastian [European Molecular Biology Laboratory, Heidelberg, Germany > Structural and Computational Biology Unit]
	Hayward, Matthew [European Molecular Biology Laboratory, Heidelberg, Germany > Structural and Computational Biology Unit]
	Becher, Dorte [Institute of Microbiology, University of Greifswald, Greifswald, Germany]
	Heintz-Buschart, Anna [Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands]
	de Beaufort, Carine [University of Luxembourg > > >]
	Bork, Peer [European Molecular Biology Laboratory, Heidelberg, Germany > Structural and Computational Biology Unit,]
	May, Patrick [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Bioinformatics Core >]
	Wilmes, Paul [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology >]
Publication date :	Dec-2022
Journal title :	Microbiome
Publisher :	BioMed Central
Peer reviewed :	Yes
Audience :	International
e-ISSN :	2049-2618
City :	London
Country :	United Kingdom
Keywords :	[en] Microbiome ; Type 1 Diabetes ; Meta-Omics
Abstract :	[en] Background: Alterations to the gut microbiome have been linked to multiple chronic diseases. However, the drivers of such changes remain largely unknown. The oral cavity acts as a major route of exposure to exogenous factors including pathogens, and processes therein may affect the communities in the subsequent compartments of the gastrointestinal tract. Here, we perform strain‑resolved, integrated meta‑genomic, transcriptomic, and proteomic analyses of paired saliva and stool samples collected from 35 individuals from eight families with multiple cases of type 1 diabetes mellitus (T1DM). Results: We identified distinct oral microbiota mostly reflecting competition between streptococcal species. More specifically, we found a decreased abundance of the commensal Streptococcus salivarius in the oral cavity of T1DM individuals, which is linked to its apparent competition with the pathobiont Streptococcus mutans. The decrease in S. salivarius in the oral cavity was also associated with its decrease in the gut as well as higher abundances in facultative anaerobes including Enterobacteria. In addition, we found evidence of gut inflammation in T1DM as reflected in the expression profiles of the Enterobacteria as well as in the human gut proteome. Finally, we were able to follow transmitted strain‑variants from the oral cavity to the gut at the individual omic levels, highlighting not only the transfer, but also the activity of the transmitted taxa along the gastrointestinal tract. Conclusions: Alterations of the oral microbiome in the context of T1DM impact the microbial communities in the lower gut, in particular through the reduction of “mouth‑to‑gut” transfer of Streptococcus salivarius. Our results indicate that the observed oral‑cavity‑driven gut microbiome changes may contribute towards the inflammatory processes involved in T1DM. Through the integration of multi‑omic analyses, we resolve strain‑variant “mouth‑to‑gut” transfer in a disease context.
Research centres :	Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group) ; Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (R. Schneider Group)
Funders :	Fonds National de la Recherche - FnR
Target :	Researchers
Permalink :	http://hdl.handle.net/10993/54243
DOI :	10.1186/s40168-022-01435-4
Other URL :	https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-022-01435-4
FnR project :	FnR ; FNR10404093 > Paul Wilmes > microCancer > Non-invasive Microbiome-derived Multi-omic Biomarkers For Early-stage Colorectal Cancer Detection > 01/01/2016 > 30/04/2019 > 2015

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Open access	s40168-022-01435-4.pdf		Publisher postprint	11.96 MB	View/Open

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Open access	40168_2022_1435_MOESM3_ESM.pdf	Supplementary figures	609.14 kB	View/Open

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