Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell  migration.

[en] Glioblastoma (GBM) is the most aggressive primary brain tumor characterized by infiltrative growth of malignant glioma cells into the surrounding brain parenchyma. In this study, our analysis of GBM patient cohorts revealed a significantly higher expression of Glycosyltransferase 8 domain containing 1 (GLT8D1) compared to normal brain tissue and could be associated with impaired patient survival. Increased in vitro expression of GLT8D1 significantly enhanced migration of two different sphere-forming GBM cell lines. By in silico analysis we predicted the 3D-structure as well as the active site residues of GLT8D1. The introduction of point mutations in the predicted active site reduced its glycosyltransferase activity in vitro and consequently impaired GBM tumor cell migration. Examination of GLT8D1 interaction partners by LC-MS/MS implied proteins associated with cytoskeleton and intracellular transport as potential substrates. In conclusion, we demonstrated that the enzymatic activity of glycosyltransferase GLT8D1 promotes GBM cell migration.

Disciplines :

Oncology

Author, co-author :

ILINA, Elena ; University of Luxembourg ; Department of Cancer Research (DoCR), Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg. ; Luxembourg Centre of Neuropathology (LCNP), 1526 Luxembourg, Luxembourg.

CIALINI, Camille ; University of Luxembourg ; Department of Cancer Research (DoCR), Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg. ; Luxembourg Centre of Neuropathology (LCNP), 1526 Luxembourg, Luxembourg.

GERLOFF, Dietlind ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine > Bioinformatics Core ; Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA.

Duarte Garcia-Escudero, Maitane; Department of Cancer Research (DoCR), Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg. ; Luxembourg Centre of Neuropathology (LCNP), 1526 Luxembourg, Luxembourg.

Jeanty, Céline; Quantitative Biology Unit, Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg.

Thézénas, Marie-Laëtitia; Quantitative Biology Unit, Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg.

Lesur, Antoine; Quantitative Biology Unit, Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg.

Puard, Vincent; Quantitative Biology Unit, Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg.

Bernardin, François; Quantitative Biology Unit, Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg.

Moter, Alina; Department of Cancer Research (DoCR), Luxembourg Institute of Health (LIH), 1526 Luxembourg, Luxembourg. ; Luxembourg Centre of Neuropathology (LCNP), 1526 Luxembourg, Luxembourg.

More authors (8 more)

External co-authors :

yes

Language :

English

Title :

Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration.

Publication date :

18 February 2022

Journal title :

iScience

eISSN :

2589-0042

Publisher :

Elsevier, Cambridge, United States - Massachusetts

Volume :

Issue :

Pages :

103842

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBilu :

since 16 February 2024

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