Reference : Automated high-throughput high-content autophagy and mitophagy analysis platform
Scientific journals : Article
Life sciences : Genetics & genetic processes
Systems Biomedicine
http://hdl.handle.net/10993/39784
Automated high-throughput high-content autophagy and mitophagy analysis platform
English
Arias, Jonathan [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Jarazo, Javier mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Walter, Jonas [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Gomez Giro, Gemma mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Forster, Julia [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Antony, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Krüger, Rejko mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Schwamborn, Jens Christian mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Life Science Research Unit >]
1-Jul-2019
Scientific Reports
Nature Publishing Group
Yes (verified by ORBilu)
International
2045-2322
London
United Kingdom
[en] Induced pluripotent stem cells ; Mitophagy
[en] Autophagic processes play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy can be affected at multiple and distinct steps of the pathway. Current analyses tools do not deliver the required detail for dissecting pathway intermediates. The development of new tools to analyze autophagic processes qualitatively and quantitatively in a more straightforward manner is required. Defining all autophagy pathway intermediates in a high-throughput manner is technologically challenging and has not been addressed yet. Here, we overcome those requirements and limitations by the developed of stable autophagy and mitophagy reporter-iPSC and the establishment of a novel high-throughput phenotyping platform utilizing automated high-content image analysis to assess autophagy and mitophagy pathway intermediates.
http://hdl.handle.net/10993/39784
also: http://hdl.handle.net/10993/42612
10.1038/s41598-019-45917-2
https://www.nature.com/articles/s41598-019-45917-2#article-info
Open Access

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