Article (Scientific journals)
Secretion of the Phosphorylated Form of S100A9 from Neutrophils Is Essential for the Proinflammatory Functions of Extracellular S100A8/A9.
Schenten, Veronique; Plancon, Sebastien; Jung, Nicolas et al.
2018In Frontiers in Immunology, 9, p. 447
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Keywords :
S100A8/PhosphoA9; Toll-like receptor 4; cytokines; inflammation; neutrophils
Abstract :
[en] S100A8 and S100A9 are members of the S100 family of cytoplasmic EF-hand Ca(2+)-binding proteins and are abundantly expressed in the cytosol of neutrophils. In addition to their intracellular roles, S100A8/A9 can be secreted in the extracellular environment and are considered as alarmins able to amplify the inflammatory response. The intracellular activity of S100A8/A9 was shown to be regulated by S100A9 phosphorylation, but the importance of this phosphorylation on the extracellular activity of S100A8/A9 has not yet been extensively studied. Our work focuses on the impact of the phosphorylation state of secreted S100A9 on the proinflammatory function of neutrophils. In a first step, we characterized the secretion of S100A8/A9 in different stimulatory conditions and investigated the phosphorylation state of secreted S100A9. Our results on neutrophil-like differentiated HL-60 (dHL-60) cells and purified human neutrophils showed a time-dependent secretion of S100A8/A9 when induced by phorbol 12-myristoyl 13-acetate and this secreted S100A9 was found in a phosphorylated form. Second, we evaluated the impact of this phosphorylation on proinflammatory cytokine expression and secretion in dHL-60 cells. Time course experiments with purified unphosphorylated or phosphorylated S100A8/A9 were performed and the expression and secretion levels of interleukin (IL)-1alpha, IL-1beta, IL-6, tumor necrosis factor alpha, CCL2, CCL3, CCL4, and CXCL8 were measured by real-time PCR and cytometry bead array, respectively. Our results demonstrate that only the phosphorylated form of the complex induces proinflammatory cytokine expression and secretion. For the first time, we provide evidence that S100A8/PhosphoS100A9 is inducing cytokine secretion through toll-like receptor 4 signaling.
Research center :
Life Sciences Research Unit (LSRU): Immune Cells and Inflammatory Diseases (ICID)
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Schenten, Veronique;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Plancon, Sebastien;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Jung, Nicolas ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Hann, Justine ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Bueb, Jean-Luc ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Bréchard, Sabrina ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Tschirhart, Eric ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Tolle, Fabrice ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
External co-authors :
no
Language :
English
Title :
Secretion of the Phosphorylated Form of S100A9 from Neutrophils Is Essential for the Proinflammatory Functions of Extracellular S100A8/A9.
Publication date :
March 2018
Journal title :
Frontiers in Immunology
ISSN :
1664-3224
Publisher :
Frontiers Research Foundation, Lausanne, Switzerland
Volume :
9
Pages :
447
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Damage Associated Molecular Patterns (DAMP)
Funders :
Research grant F1R-LSC-PUL-13DAMP from the University of Luxembourg
Available on ORBilu :
since 10 April 2018

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