Article (Scientific journals)
Platelet mitochondrial membrane potential in Parkinson's disease.
ANTONY, Paul; BOYD, Olga; TREFOIS, Christophe et al.
2015In Annals of Clinical and Translational Neurology, 2 (1), p. 67-73
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Abstract :
[en] OBJECTIVE: Mitochondrial dysfunction is a hallmark of idiopathic Parkinson's disease (IPD), which has been reported not to be restricted to striatal neurons. However, studies that analyzed mitochondrial function at the level of selected enzymatic activities in peripheral tissues have produced conflicting data. We considered the electron transport chain as a complex system with mitochondrial membrane potential as an integrative indicator for mitochondrial fitness. METHODS: Twenty-five IPD patients (nine females; mean disease duration, 6.2 years) and 16 healthy age-matched controls (12 females) were recruited. Live platelets were purified using magnetic-activated cell sorting (MACS) and single-cell data on mitochondrial membrane potential (Deltapsi) were measured by cytometry and challenged with a protonophore agent. RESULTS: Functional mitochondrial membrane potential was detected in all participants. The challenge test reduced the membrane potential in all IPD patients and controls (P < 0.001). However, the response to the challenge was not significantly different between patients and controls. INTERPRETATION: While the reported protonophore challenge assay is a valid marker of overall mitochondrial function in live platelets, intact mitochondrial membrane potential in platelets derived from IPD patients suggests that presumed mitochondrial enzymatic deficiencies are compensable in this cell type. In consequence, mitochondrial membrane potential in platelets cannot be used as a diagnostic biomarker for nonstratified IPD but should be further explored in potential Parkinson's disease subtypes and tissues with higher energy demands.
Disciplines :
Human health sciences: Multidisciplinary, general & others
Author, co-author :
ANTONY, Paul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
BOYD, Olga ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
TREFOIS, Christophe ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Ammerlaan, Wim
OSTASZEWSKI, Marek  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Baumuratov, Aidos S.
Longhino, Laura
Antunes, Laurent
Koopman, Werner
BALLING, Rudi ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Diederich, Nico J.
External co-authors :
yes
Language :
English
Title :
Platelet mitochondrial membrane potential in Parkinson's disease.
Publication date :
2015
Journal title :
Annals of Clinical and Translational Neurology
eISSN :
2328-9503
Publisher :
Wiley, Hoboken, United States - New Jersey
Volume :
2
Issue :
1
Pages :
67-73
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 13 May 2016

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