Article (Scientific journals)
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
Neumann, Beate; Walter, Thomas; Heriche, Jean-Karim et al.
2010In Nature, 464 (7289), p. 721-727
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Abstract :
[en] Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the similar to 21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.
Disciplines :
Biochemistry, biophysics & molecular biology
Identifiers :
UNILU:UL-ARTICLE-2012-053
Author, co-author :
Neumann, Beate
Walter, Thomas
Heriche, Jean-Karim
Bulkescher, Jutta
Erfle, Holger
Conrad, Christian
Rogers, Phill
Poser, Ina
Held, Michael
Liebel, Urban
Cetin, Cihan
Sieckmann, Frank
Pau, Gregoire
Kabbe, Rolf
Wuensche, Annelie
Satagopam, Venkata ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) ; European Molecular Biology Laboratory - EMBL
Schmitz, Michael H. A.
Chapuis, Catherine
Gerlich, Daniel W.
Schneider, Reinhard ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) ; European Molecular Biology Laboratory - EMBL
Eils, Roland
Huber, Wolfgang
Peters, Jan-Michael
Hyman, Anthony A.
Durbin, Richard
Pepperkok, Rainer
Ellenberg, Jan
More authors (17 more) Less
External co-authors :
yes
Language :
English
Title :
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
Publication date :
2010
Journal title :
Nature
ISSN :
1476-4687
Publisher :
Nature Publishing Group, Basingstoke, United Kingdom
Volume :
464
Issue :
7289
Pages :
721-727
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 26 July 2014

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