chemoradiotherapy; head and neck cancer; metagenomic sequencing; oral microbiome; radiation‐induced oral mucositis; Humans; Male; Female; Middle Aged; Aged; Metagenomics/methods; Saliva/microbiology; Metagenome; Prospective Studies; Adult; Bacteria/genetics; Bacteria/classification; Microbiota/radiation effects; Microbiota/genetics; Chemoradiotherapy/adverse effects; Squamous Cell Carcinoma of Head and Neck/therapy; Squamous Cell Carcinoma of Head and Neck/microbiology; Head and Neck Neoplasms/therapy; Head and Neck Neoplasms/microbiology; Mouth/microbiology; Case-Control Studies; Head and Neck Neoplasms; Metagenomics; Microbiota; Mouth; Saliva; Squamous Cell Carcinoma of Head and Neck; Oncology; Radiology, Nuclear Medicine and Imaging; Cancer Research
Abstract :
[en] [en] BACKGROUND: We explored the interaction between the oral microbiome and the development of radiation-induced mucositis in patients with head and neck squamous cell cancer (HNSCC) undergoing chemoradiotherapy (CRT). We prospectively studied the oral microbiome and compared it to healthy controls. Additionally, we compared patients with low-grade (LGM) vs. high-grade mucositis (HGM).
METHODS: Ten HNSCC patients scheduled for CRT were included. Saliva samples were characterized prior to, during, and nine months after CRT using metagenomic sequencing. We similarly characterized samples from seven healthy controls. We assessed alpha and beta diversity and examined abundances at different taxonomic levels between (sub)groups.
RESULTS: Patients exhibited significantly reduced alpha diversity compared to controls at all times (p ⟨ 0.05). Differential abundance of taxa between patients and controls was observed at baseline. In patients, the relative abundance of Staphylococcus aureus and Escherichia coli increased significantly during CRT. Capnocytophaga spp. was associated with the definitive CRT patients' subgroup. At baseline, two fungal families (Melampsoraceae and Herpotrichiellaceaea) were more abundant in patients who later developed HGM. No differentially abundant taxa were found between LGM vs. HGM during irradiation.
CONCLUSION: Our findings support the hypothesis that CRT, as well as HNSCC itself, influences the composition of the oral microbiome. Microbial markers found in patients who later developed HGM should be evaluated using independent cohorts to qualify their specific biomarker potential.
Disciplines :
Microbiology
Author, co-author :
Torozan, Dominique A ; Department of Radiotherapy and Radiation Oncology, University Hospital, Saarland University, Homburg, Germany
RUMP, Kirsten ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Clinical and Translational Informatics
WILMES, Paul ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology
Fleckenstein, Jochen; Department of Radiotherapy and Radiation Oncology, University Hospital, Saarland University, Homburg, Germany ; Department of Radiotherapy, Westpfalz-Klinikum, Kaiserslautern, Germany
SCHNEIDER, Jochen ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Medical Translational Research ; Department of Internal Medicine II, Gastroenterology, Hepatology, Endocrinology and Diabetology, University Hospital, Saarland University, Homburg, Germany
External co-authors :
yes
Language :
English
Title :
Metagenomic Profiling of Oral Microbiome Dynamics During Chemoradiotherapy in Head and Neck Squamous Cell Carcinoma Patients.
H2020 - 863664 - ExpoBiome - Deciphering the impact of exposures from the gut microbiome-derived molecular complex in human health and disease
Funders :
H2020 European Research Council European Union
Funding text :
Funding: This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement number 863664).
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