Nerve Tissue Proteins; Proteome; Animals; Cerebrum/metabolism; Chromatography, Liquid; Gray Matter/metabolism; Humans; Male; Mass Spectrometry; Mice; Nerve Tissue Proteins/metabolism; Proteomics/methods; Synaptosomes/metabolism; White Matter/metabolism; Cerebrum; Gray Matter; Proteomics; Synaptosomes; White Matter; Neuroscience (all); General Neuroscience
Abstract :
[en] • Protocol for quantitative proteomics of nitrosylation on synaptosomal proteins. • Identification of endogenous nitrosylation independent of induction by NO donors. • Use of iodoTMT sixplex mass tags for stable labeling, enrichment, identification, and multiplex quantitation. • Applicable on low amounts of sample material of mouse and human brain tissue. [en] [Figure: see text]
Disciplines :
Neurology
Author, co-author :
Wijasa, Teodora Stella; German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany
Sylvester, Marc; Institute of Biochemistry and Molecular Biology, University of Bonn, Germany
Brocke-Ahmadinejad, Nahal; Institute of Biochemistry and Molecular Biology, University of Bonn, Germany
Kummer, Markus P; Department of Neurodegenerative Diseases & Gerontopsychiatry, University Hospital Bonn, Bonn, Germany
Brosseron, Frederic; German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany
Gieselmann, Volkmar; Institute of Biochemistry and Molecular Biology, University of Bonn, Germany
HENEKA, Michael ; German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany, Department of Neurodegenerative Diseases & Gerontopsychiatry, University Hospital Bonn, Bonn, Germany. Electronic address: michael.heneka@ukb.uni-bonn.de
External co-authors :
yes
Language :
English
Title :
Proteome profiling of s-nitrosylated synaptosomal proteins by isobaric mass tags.
German Center for Neurodegenerative Diseases (DZNE e.V.) German Research Council (DFG, Klinische Forscherguppe 177, TP4) Institute of Biochemistry and Molecular Biology at the Rheinische Friedrich-Wilhelms University, European Union's Seventh Framework Programme (FP7/2007-2013) EU-IMI
Funding text :
This work was funded by the German Center for Neurodegenerative Diseases (DZNE e.V.) within the Helmholtz Association, by the German Research Council (DFG, Klinische Forscherguppe 177, TP4) , the Institute of Biochemistry and Molecular Biology at the Rheinische Friedrich-Wilhelms University, European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement no. HEALTH-F2-2011-278850 (INMiND) and the EU-IMI program no. 115568 (AETIONOMY). Michael T. Heneka is a member of the Cluster of Excellence “Immunosensation”.
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