Vaccination with (1-11)E2 in alum efficiently induces an antibody response to β-amyloid without affecting brain β-amyloid load and microglia activation in 3xTg mice.
Vaccination with (1-11)E2 in alum efficiently induces an antibody response to β-amyloid without affecting brain β-amyloid load and microglia activation in 3xTg mice.pdf
[en] Immunization against β-amyloid (Aβ) is pursued as a possible strategy for the prevention of Alzheimer's disease (AD). In clinical trials, Aβ 1-42 proved poorly immunogenic and caused severe adverse effects; therefore, safer and more immunogenic candidate vaccines are needed. Multimeric protein (1-11)E2 is able to induce an antibody response to Aβ, immunological memory, and IL-4 production, with no concomitant anti-Aβ T cell response. Antisera recognize Aβ oligomers, protofibrils, and fibrils. In this study, we evaluated the effect of prophylactic immunization with three doses of (1-11)E2 in alum in the 3xTg mouse model of AD. Immunization with (1-11)E2 efficiently induced anti-Aβ antibodies, but afforded no protection against Aβ accumulation and neuroinflammation. The identification of the features of the anti-Aβ immune response that correlate with the ability to prevent Aβ accumulation remains an open problem that deserves further investigation.
Disciplines :
Neurology
Author, co-author :
Mantile, Francesca; Istituto di Genetica e Biofisica Adriano Buzzati Traverso (IGB), CNR, Naples, Italy
Capasso, Angelo; Istituto di Genetica e Biofisica Adriano Buzzati Traverso (IGB), CNR, Naples, Italy
Villacampa, Nadia; Deutsches Zentrum fur Neurodegenerative Erkrankungen (DZNE), Bonn, Germany
Donnini, Maria; Istituto di Genetica e Biofisica Adriano Buzzati Traverso (IGB), CNR, Naples, Italy
Liguori, Giovanna L; Istituto di Genetica e Biofisica Adriano Buzzati Traverso (IGB), CNR, Naples, Italy
Constantin, Gabriela; Università degli Studi di Verona Dipartimento di Medicina Sezione di Patologia Generale, Verona, Italy
De Berardinis, Piergiuseppe ✱; Istituto di Biochimica e Biologia Cellulare (IBBC), CNR, Naples, Italy
HENEKA, Michael ✱; Deutsches Zentrum fur Neurodegenerative Erkrankungen (DZNE), Bonn, Germany
Prisco, Antonella ✱; Istituto di Genetica e Biofisica Adriano Buzzati Traverso (IGB), CNR, Naples, Italy. antonella.prisco@igb.cnr.it
✱ These authors have contributed equally to this work.
External co-authors :
yes
Language :
English
Title :
Vaccination with (1-11)E2 in alum efficiently induces an antibody response to β-amyloid without affecting brain β-amyloid load and microglia activation in 3xTg mice.
Publication date :
May 2021
Journal title :
Aging Clinical and Experimental Research
ISSN :
1594-0667
eISSN :
1720-8319
Publisher :
Springer Science and Business Media Deutschland GmbH, Germany
Consiglio Nazionale delle Ricerche EU Joint Programme – Neurodegenerative Disease Research H2020 European Research Council EMBO European Federation of Immunological Societies
Funding text :
This work was supported by the National Research Council of Italy (CNR), Research Project “Aging: molecular and technological innovations for improving the health of the elderly population” (Prot. MIUR 2867 25.11.2011) (to PDB and AP), by JPND project DACAPO-AD 2015 JPco-fuND call (to MTH), by PO FESR 2014–2020 “SATIN” from Regione Campania (to PDB and GLL), by the European Union’s Horizon 2020 research and innovation programme Grant 801338 (to GLL), and by European Research Council (ERC) Grant 695714 IMMUNOALZHEIMER (to GC). FM acknowledges fellowships from EMBO and EFIS.This work was supported by the National Research Council of Italy (CNR), Research Project ?Aging: molecular and technological innovations for improving the health of the elderly population? (Prot. MIUR 2867 25.11.2011) (to PDB and AP), by JPND project DACAPO-AD 2015 JPco-fuND call (to MTH), by PO FESR 2014?2020 ?SATIN? from Regione Campania (to PDB and GLL), by the European Union?s Horizon 2020 research and innovation programme Grant 801338 (to GLL), and by European Research Council (ERC) Grant 695714 IMMUNOALZHEIMER (to GC). FM acknowledges fellowships from EMBO and EFIS.
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