Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).

Allergy and Immunology/standards; Cell Separation/methods; Cell Separation/standards; Consensus; Flow Cytometry/methods; Flow Cytometry/standards; Humans; Phenotype; Allergy and Immunology; Cell Separation; Flow Cytometry; Immunology and Allergy; Immunology

Abstract :

[en] These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Disciplines :

Immunology & infectious disease

Author, co-author :

Cossarizza, Andrea; Department of Medical and Surgical Sciences for Children and Adults, Univ. of Modena and Reggio Emilia School of Medicine, Modena, Italy

Chang, Hyun-Dong; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany

Radbruch, Andreas; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany

Acs, Andreas; Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany

Adam, Dieter; Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany

Adam-Klages, Sabine; Institut für Transfusionsmedizin, Universitätsklinik Schleswig-Holstein, Kiel, Germany

Agace, William W; Mucosal Immunology group, Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark ; Immunology Section, Lund University, Lund, Sweden

Aghaeepour, Nima; Departments of Anesthesiology, Pain and Perioperative Medicine, Biomedical Data Sciences, and Pediatrics, Stanford University, Stanford, CA, USA

Akdis, Mübeccel; Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland

Allez, Matthieu; Université de Paris, Institut de Recherche Saint-Louis, INSERM U1160, and Gastroenterology Department, Hôpital Saint-Louis - APHP, Paris, France

More authors (426 more)

External co-authors :

yes

Language :

English

Title :

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).

Publication date :

October 2019

Journal title :

European Journal of Immunology

ISSN :

0014-2980

eISSN :

1521-4141

Publisher :

Wiley-VCH Verlag, Germany

Volume :

Issue :

Pages :

1457 - 1973

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.201970107

Funding text :

Ramon Bellmàs Sanz, Jenny Kühne, and Christine S. Falk thank Jana Keil and Kerstin Daemen for excellent technical support. The work was funded by the Germany Research Foundation CRC738/B3 (CSF).Larissa Nogueira Almeida was supported by DFG research grant MA 2273/14‐1. Rudolf A. Manz was supported by the Excellence Cluster “Inflammation at Interfaces” (EXC 306/2).Susanne Hartmann and Friederike Ebner were supported by the German Research Foundation (GRK 2046).Edwin van der Pol and Paola Lanuti acknowledge Aleksandra Gąsecka M.D. for excellent experimental support and Dr. Rienk Nieuwland for textual suggestions. This work was supported by the Netherlands Organisation for Scientific Research – Domain Applied and Engineering Sciences (NWO‐TTW), research program VENI 15924.Hans Minderman was supported by NIH R50CA211108.Work in the laboratory of Dieter Adam is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Projektnummer 125440785 – SFB 877, Project B2.Christian Kukat acknowledges support from the ISAC SRL Emerging Leaders program.This work was funded by the Deutsche Forschungsgemeinschaft through the grant TRR130 (project P11 and C03) to Thomas H. Winkler.The Immunology Database and Analysis Portal (ImmPort) system provides an archive of immunology research data generated by investigators mainly funded through the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), and Division of Allergy, Immunology, and Transplantation (DAIT). It is an extensive data warehouse containing an integration of experimental and clinical trial data generated by dozens of assay types, including 131 FCM and 24 CyTOF data sets. In addition, the ImmPort system also provides data analysis tools and it contains implicit knowledge and ‘‘best practices’’ for clinical and genomic studies in the form of ∼50 templates for data deposition, management, and dissemination. ImmPort has been developed under the Bioinformatics Integration Support Contract (BISC) by the Northrop Grumman Information Technology Health Solutions team for the NIH NIAID/DAIT. If your research funding comes from this source and you are generating immunology data, you should deposit it in ImmPort. Immport's support for different data types can be another reason to choose it if you are generating FCM data as well as data from different types of assays. A (free) registration and approval by DAIT is required in order to deposit and access data from ImmPort. A potential drawback of using this resource is that there can be a long delay in publication of deposited datasets.The work by the Mei laboratory was supported by German Research Foundation Grant ME 3644/5‐1 and TRR130 TP24, the German Rheumatism Research Centre Berlin, European Union Innovative Medicines Initiative ‐ Joint Undertaking ‐ RTCure Grant Agreement 777357, the Else Kröner‐Fresenius‐Foundation, German Federal Ministry of Education and Research e:Med sysINFLAME Program Grant 01ZX1306B and KMU‐innovativ “InnoCyt”, and the Leibniz Science Campus for Chronic Inflammation ( http://www.chronische-entzuendung.org ).Petra Hoffmann, Andrea Hauser, and Matthias Edinger thank BD Biosciences®, San José, CA, USA, and SKAN AG, Bale, Switzerland for fruitful cooperation during the development, construction, and installation of the GMP‐compliant cell sorting equipment and the Bavarian Immune Therapy Network (BayImmuNet) for financial support.John Trowsdale received funding from the European Research Council under the European Union's Horizon 2020 research and innovation program (Grant Agreement 695551).

Data Set :

10.1002/eji.201970107

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