Article (Scientific journals)
Recombinant interleukin-24 lacks apoptosis-inducing properties in melanoma cells
Kreis, Stephanie; Philippidou, Demetra; Margue, Christiane et al.
2007In PLoS ONE, 2 (12), p. 1300
Peer Reviewed verified by ORBi
 

Files


Full Text
Recombinant Interleukin_2007_ PlosONE.pdf
Publisher postprint (760.77 kB)
Download

All documents in ORBilu are protected by a user license.

Send to



Details



Abstract :
[en] IL-24, also known as melanoma differentiation antigen 7 (mda-7), is a member of the IL-10 family of cytokines and is mainly produced by Th(2) cells as well as by activated monocytes. Binding of IL-24 to either of its two possible heterodimeric receptors IL-20R1/IL-20R2 and IL-22R/IL-20R2 activates STAT3 and/or STAT1 in target tissues such as lung, testis, ovary, keratinocytes and skin. To date, the physiological properties of IL-24 are still not well understood but available data suggest that IL-24 affects epidermal functions by increasing proliferation of dermal cells. In stark contrast to its "normal" and physiological behaviour, IL-24 has been reported to selectively and efficiently kill a vast variety of cancer cells, especially melanoma cells, independent of receptor expression and Jak-STAT signalling. These intriguing properties have led to the development of adenovirally-expressed IL-24, which is currently being evaluated in clinical trials. Using three different methods, we have analysed a large panel of melanoma cell lines with respect to IL-24 and IL-24 receptor expression and found that none of the investigated cell lines expressed sufficient amounts of functional receptor pairs and therefore did not react to IL-24 stimulation with Jak/STAT activation. Results for three cell lines contrasted with previous studies, which reported presence of IL-24 receptors and activation of STAT3 following IL-24 stimulation. Furthermore, evaluating four different sources and modes of IL-24 administration (commercial recombinant IL-24, bacterially expressed GST-IL-24 fusion protein, IL-24 produced from transfected Hek cells, transiently over-expressed IL-24) no induction or increase in cell death was detected when compared to appropriate control treatments. Thus, we conclude that the cytokine IL-24 itself has no cancer-specific apoptosis-inducing properties in melanoma cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Identifiers :
UNILU:UL-ARTICLE-2008-696
Author, co-author :
Kreis, Stephanie ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Philippidou, Demetra ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Margue, Christiane  
Rolvering, Catherine ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Haan, Claude ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Dumoutier, Laure;  Université Catholique de Louvain - UCL > Ludwig Institute for Cancer Research
Renauld, Jean-Christophe;  Université Catholique de Louvain - UCL > Ludwig Institute for Cancer Research
Behrmann, Iris ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
External co-authors :
yes
Language :
English
Title :
Recombinant interleukin-24 lacks apoptosis-inducing properties in melanoma cells
Publication date :
2007
Journal title :
PLoS ONE
ISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
2
Issue :
12
Pages :
e1300
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
Available on ORBilu :
since 19 August 2013

Statistics


Number of views
76 (6 by Unilu)
Number of downloads
45 (1 by Unilu)

Scopus citations®
 
29
Scopus citations®
without self-citations
27
OpenCitations
 
28
WoS citations
 
29

Bibliography


Similar publications



Contact ORBilu