Reference : Deep sncRNA-seq of the PPMI cohort to study Parkinson’s disease progression
E-prints/Working papers : Already available on another site
Life sciences : Biochemistry, biophysics & molecular biology
Systems Biomedicine
Deep sncRNA-seq of the PPMI cohort to study Parkinson’s disease progression
Kern, Fabian [Saarland University > Clinical Bioinformatics]
Fehlmann, Tobias [Saarland University > Clinical Bioinformatics]
Violich, Ivo [University of Southern California > Keck School of Medicine]
Alsop, Eric [Translational Genomics Research Institute > Neurogenomics Division]
Hutchins, Elizabeth [Translational Genomics Research Institute > Neurogenomics Division]
Kahraman, Mustafa [> >]
Grammes, Nadja Liddy [> >]
Guimarães, Pedro [> >]
Backes, Christina [> >]
Poston, Kathleen [> >]
Casey, Bradford [> >]
Balling, Rudolf mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Geffers, Lars [Luxembourg Institute of Health - LIH]
Krüger, Rejko mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
Galasko, Douglas [> >]
Mollenhauer, Brit [> >]
Meese, Eckart [> >]
Wyss-Coray, Tony [> >]
Craig, David Wesley [> >]
Van Keuren-Jensen, Kendall [> >]
Keller, Andreas [> >]
Cold Spring Harbor Laboratory
[en] RNAs ; Parkinson’s diseases ; sncRNA-seq
[en] Coding and non-coding RNAs have diagnostic and prognostic importance in Parkinson’s diseases (PD). We studied circulating small non-coding RNAs (sncRNAs) in 7, 003 samples from two longitudinal PD cohorts (Parkinson’s Progression Marker Initiative (PPMI) and Luxembourg Parkinson’s Study (NCER-PD)) and modelled their influence on the transcriptome. First, we sequenced sncRNAs in 5, 450 blood samples of 1, 614 individuals in PPMI. The majority of 323 billion reads (59 million reads per sample) mapped to miRNAs. Other covered RNA classes include piRNAs, rRNAs, snoRNAs, tRNAs, scaRNAs, and snRNAs. De-regulated miRNAs were associated with the disease and disease progression and occur in two distinct waves in the third and seventh decade of live. Originating mostly from a characteristic set of immune cells they resemble a systemic inflammation response and mitochondrial dysfunction, two hallmarks of PD. By profiling 1, 553 samples from 1, 024 individuals in the NCER-PD cohort using an independent technology, we validate relevant findings from the sequencing study. Finally, network analysis of sncRNAs and transcriptome sequencing of the original cohort identified regulatory modules emerging in progressing PD patients.Competing Interest StatementThe authors have declared no competing interest.
Michael J. Fox Foundation
Researchers ; Professionals ; Students

File(s) associated to this reference

Fulltext file(s):

Open access
Deep_sncRNA-seq.full.pdfPublisher postprint8.54 MBView/Open

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.