Reference : Induced pluripotent stem cell line (LCSBi001-A) derived from a patient with Parkinson...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/10993/43432

Title :	Induced pluripotent stem cell line (LCSBi001-A) derived from a patient with Parkinson's disease carrying the p.D620N mutation in VPS35
Language :	English
Author, co-author :	Larsen, Simone [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Hanss, Zoé [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Cruciani, Gérald [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Massart, François [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Barbuti, Peter [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Mellick, George [Griffith University > Griffith Institute for Drug Discovery]
	Krüger, Rejko [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Publication date :	4-Apr-2020
Journal title :	Stem Cell Research
Publisher :	Elsevier
Peer reviewed :	Yes (verified by ORBilu)
Audience :	International
ISSN :	1873-5061
e-ISSN :	1876-7753
City :	Amsterdam
Country :	Netherlands
Keywords :	[en] iPSC ; VPS35 ; Parkinson
Abstract :	[en] Fibroblasts were obtained from a 76 year-old man diagnosed with Parkinson's disease (PD). The disease is caused by a heterozygous p.D620N mutation in VPS35. Induced pluripotent stem cells (iPSCs) were generated using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific). The presence of the c.1858G > A base exchange in exon 15 of VPS35 was confirmed by Sanger sequencing. The iPSCs are free of genomically integrated reprogramming genes, express pluripotency markers, display in vitro differentiation potential to the three germ layers and have karyotypic integrity. Our iPSC line will be useful for studying the impact of the p.D620N mutation in VPS35 in vitro.
Funders :	Fonds National de la Recherche - FnR ; H2020
Target :	Researchers
Permalink :	http://hdl.handle.net/10993/43432
DOI :	10.1016/j.scr.2020.101776
Other URL :	https://www.sciencedirect.com/science/article/pii/S1873506120300805?via%3Dihub
Framework Programme / European Project :	H2020 ; 692320 - CENTRE-PD - TWINNING for a Comprehensive Clinical Centre for the Diagnosis and Treatment of Parkinson's Disease
FnR project :	FnR ; FNR6682797 > Rejko Krüger > Endophenotypes in Neurodegeneration > Comprehensive assessment of endophenotypes in neurodegenerative diseases - translating impaired molecular signalling pathways into novel therapeutic strategies for Parkinson’s disease > 01/06/2014 > 31/05/2019 > 2013

	File(s) associated to this reference
	Fulltext file(s): File Commentary Version Size Access Open access Larsen - SCR 2020.pdf Publisher postprint 1.08 MB View/Open

All documents in ORBi^lu are protected by a user license.

 

University of Luxembourg Library |  Feedback |  Legal notices Site Map