Article (Scientific journals)
A Conserved Mito-Cytosolic Translational Balance Links Two Longevity Pathways.
Molenaars, Marte; Janssens, Georges E.; Williams, Evan et al.
2020In Cell Metabolism, 31 (3), p. 549-563.e7
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Keywords :
ATF4; aging; cytosolic translation; doxycycline; longevity; mitochondrial translation; polysome; ribosomes; translational balance; translational efficiency
Abstract :
[en] Slowing down translation in either the cytosol or the mitochondria is a conserved longevity mechanism. Here, we found a non-interventional natural correlation of mitochondrial and cytosolic ribosomal proteins (RPs) in mouse population genetics, suggesting a translational balance. Inhibiting mitochondrial translation in C. elegans through mrps-5 RNAi repressed cytosolic translation. Transcriptomics integrated with proteomics revealed that this inhibition specifically reduced translational efficiency of mRNAs required in growth pathways while increasing stress response mRNAs. The repression of cytosolic translation and extension of lifespan from mrps-5 RNAi were dependent on atf-5/ATF4 and independent from metabolic phenotypes. We found the translational balance to be conserved in mammalian cells upon inhibiting mitochondrial translation pharmacologically with doxycycline. Lastly, extending this in vivo, doxycycline repressed cytosolic translation in the livers of germ-free mice. These data demonstrate that inhibiting mitochondrial translation initiates an atf-5/ATF4-dependent cascade leading to coordinated repression of cytosolic translation, which could be targeted to promote longevity.
Disciplines :
Genetics & genetic processes
Author, co-author :
Molenaars, Marte
Janssens, Georges E.
Williams, Evan  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Jongejan, Aldo
Lan, Jiayi
Rabot, Sylvie
Joly, Fatima
Moerland, Perry D.
Schomakers, Bauke V.
Lezzerini, Marco
Liu, Yasmine J.
McCormick, Mark A.
Kennedy, Brian K.
van Weeghel, Michel
van Kampen, Antoine H. C.
Aebersold, Ruedi
MacInnes, Alyson W.
Houtkooper, Riekelt H.
More authors (8 more) Less
External co-authors :
yes
Language :
English
Title :
A Conserved Mito-Cytosolic Translational Balance Links Two Longevity Pathways.
Publication date :
2020
Journal title :
Cell Metabolism
ISSN :
1932-7420
Publisher :
Cell Press, United States
Volume :
31
Issue :
3
Pages :
549-563.e7
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2020 Elsevier Inc. All rights reserved.
Available on ORBilu :
since 11 May 2020

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