Reference : A Conserved Mito-Cytosolic Translational Balance Links Two Longevity Pathways.
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/10993/43145
A Conserved Mito-Cytosolic Translational Balance Links Two Longevity Pathways.
English
Molenaars, Marte [> >]
Janssens, Georges E. [> >]
Williams, Evan mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Jongejan, Aldo [> >]
Lan, Jiayi [> >]
Rabot, Sylvie [> >]
Joly, Fatima [> >]
Moerland, Perry D. [> >]
Schomakers, Bauke V. [> >]
Lezzerini, Marco [> >]
Liu, Yasmine J. [> >]
McCormick, Mark A. [> >]
Kennedy, Brian K. [> >]
van Weeghel, Michel [> >]
van Kampen, Antoine H. C. [> >]
Aebersold, Ruedi [> >]
MacInnes, Alyson W. [> >]
Houtkooper, Riekelt H. [> >]
2020
Cell metabolism
31
3
549-563.e7
Yes (verified by ORBilu)
1550-4131
1932-7420
United States
[en] ATF4 ; aging ; cytosolic translation ; doxycycline ; longevity ; mitochondrial translation ; polysome ; ribosomes ; translational balance ; translational efficiency
[en] Slowing down translation in either the cytosol or the mitochondria is a conserved longevity mechanism. Here, we found a non-interventional natural correlation of mitochondrial and cytosolic ribosomal proteins (RPs) in mouse population genetics, suggesting a translational balance. Inhibiting mitochondrial translation in C. elegans through mrps-5 RNAi repressed cytosolic translation. Transcriptomics integrated with proteomics revealed that this inhibition specifically reduced translational efficiency of mRNAs required in growth pathways while increasing stress response mRNAs. The repression of cytosolic translation and extension of lifespan from mrps-5 RNAi were dependent on atf-5/ATF4 and independent from metabolic phenotypes. We found the translational balance to be conserved in mammalian cells upon inhibiting mitochondrial translation pharmacologically with doxycycline. Lastly, extending this in vivo, doxycycline repressed cytosolic translation in the livers of germ-free mice. These data demonstrate that inhibiting mitochondrial translation initiates an atf-5/ATF4-dependent cascade leading to coordinated repression of cytosolic translation, which could be targeted to promote longevity.
http://hdl.handle.net/10993/43145
Copyright (c) 2020 Elsevier Inc. All rights reserved.

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