Article (Scientific journals)
Large-scale validation of miRNAs by disease association, evolutionary conservation and pathway activity.
Keller, Andreas; Fehlmann, Tobias; Laufer, Thomas et al.
2018In RNA Biology
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Keywords :
NGS; evolution of miRNAs; microRNA validation; microarray; northern blot; target pathways
Abstract :
[en] The validation of microRNAs (miRNAs) identified by next generation sequencing involves amplification-free and hybridization-based detection of transcripts as criteria for confirming valid miRNAs. Since respective validation is frequently not performed, miRNA repositories likely still contain a substantial fraction of false positive candidates while true miRNAs are not stored in the repositories yet. Especially if downstream analyses are performed with these candidates (e.g. target or pathway prediction), the results may be misleading. In the present study, we evaluated 558 mature miRNAs from miRBase and 1,709 miRNA candidates from next generation sequencing experiments by amplification-free hybridization and investigated their distributions in patients with various disease conditions. Notably, the most significant miRNAs in diseases are often not contained in the miRBase. However, these candidates are evolutionary highly conserved. From the expression patterns, target gene and pathway analyses and evolutionary conservation analyses, we were able to shed light on the complexity of miRNAs in humans. Our data also highlight that a more thorough validation of miRNAs identified by next generation sequencing is required. The results are available in miRCarta ( https://mircarta.cs.uni-saarland.de ).
Disciplines :
Neurology
Author, co-author :
Keller, Andreas
Fehlmann, Tobias
Laufer, Thomas
Backes, Christina
Kahramann, Mustafa
Alles, Julia
Fischer, Ulrike
Minet, Marie
Ludwig, Nicole
Kern, Fabian
Kehl, Tim
Galata, Valentina
Dusterloh, Aneta
Schrors, Hannah
Kohlhaas, Jochen
Bals, Robert
Huwer, Hanno
GEFFERS, Lars ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
KRÜGER, Rejko ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
BALLING, Rudi ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Lenhof, Hans-Peter
Meese, Eckart
More authors (12 more) Less
External co-authors :
yes
Language :
English
Title :
Large-scale validation of miRNAs by disease association, evolutionary conservation and pathway activity.
Publication date :
26 December 2018
Journal title :
RNA Biology
ISSN :
1555-8584
Publisher :
Landes Bioscience, United States
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
Available on ORBilu :
since 21 January 2020

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