Reference : Determination of the rheological properties of Matrigel for optimum seeding condition...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/41621
Determination of the rheological properties of Matrigel for optimum seeding conditions in microfluidic cell cultures
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Kane, K. I. W. [Baller, J (Reprint Author), Univ Luxembourg, Lab Phys Adv Mat, Phys Mat Sci Res Unit, 162A Ave Faiencerie, L-1511 Luxembourg, Luxembourg. Kane, K. I. W.]
Moreno, E. Lucumi [Moreno, E. Lucumi]
Lehr, Claudius Moritz mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit]
Hachi, S. [Fleming, R. M. T., Univ Luxembourg, Luxembourg Ctr Syst Biomed, 7 Ave Hauts Fourneaux, L-4362 Esch Sur Alzette, Luxembourg. Lehr, C. M.]
Dannert, R. [Dannert, R.]
Sanctuary, Roland André mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit]
Wagner, Christian mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit]
Fleming, R. M. T. [Baller, J., Univ Luxembourg, Lab Phys Adv Mat, Phys Mat Sci Res Unit, 162A Ave Faiencerie, L-1511 Luxembourg, Luxembourg. Wagner, C., Saarland Univ, Dept Expt Phys, Campus E2-6, D-66123 Saarbrucken, Germany.]
Baller, Jörg mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit]
2018
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[en] Hydrogels are increasingly used as a surrogate extracellular matrix in three-dimensional cell culture systems, including microfluidic cell culture. Matrigel is a hydrogel of natural origin widely used in cell culture, particularly in the culture of stem cell-derived cell lines. The use of Matrigel as a surrogate extracellular matrix in microfluidic systems is challenging due to its biochemical, biophysical, and biomechanical properties. Therefore, understanding and characterising these properties is a prerequisite for optimal use of Matrigel in microfluidic systems. We used rheological measurements and particle image velocimetry to characterise the fluid flow dynamics of liquefied Matrigel during loading into a three-dimensional microfluidic cell culture device. Using fluorescence microscopy and fluorescent beads for particle image velocimetry measurements (velocity profiles) in combination with classical rheological measurements of Matrigel (viscosity versus shear rate), we characterised the shear rates experienced by cells in a microfluidic device for three-dimensional cell culture. This study provides a better understanding of the mechanical stress experienced by cells, during seeding of a mixture of hydrogel and cells, into three-dimensional microfluidic cell culture devices. (C) 2018 Author(s).
http://hdl.handle.net/10993/41621
10.1063/1.5067382
Article

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