Article (Scientific journals)
Integrated multi-omics of the human gut microbiome in a case study of familial type 1 diabetes.
Heintz, Anna; May, Patrick; Laczny, Cedric C. et al.
2016In Nature Microbiology, 2, p. 16180
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Abstract :
[en] The gastrointestinal microbiome is a complex ecosystem with functions that shape human health. Studying the relationship between taxonomic alterations and functional repercussions linked to disease remains challenging. Here, we present an integrative approach to resolve the taxonomic and functional attributes of gastrointestinal microbiota at the metagenomic, metatranscriptomic and metaproteomic levels. We apply our methods to samples from four families with multiple cases of type 1 diabetes mellitus (T1DM). Analysis of intra- and inter-individual variation demonstrates that family membership has a pronounced effect on the structural and functional composition of the gastrointestinal microbiome. In the context of T1DM, consistent taxonomic differences were absent across families, but certain human exocrine pancreatic proteins were found at lower levels. The associated microbial functional signatures were linked to metabolic traits in distinct taxa. The methodologies and results provide a foundation for future large-scale integrated multi-omic analyses of the gastrointestinal microbiome in the context of host-microbe interactions in human health and disease.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
- Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (R. Schneider Group)
- Luxembourg Centre for Systems Biomedicine (LCSB): Medical Translational Research (J. Schneider Group)
Disciplines :
Microbiology
Author, co-author :
Heintz, Anna ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
May, Patrick  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Laczny, Cedric C.;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Lebrun, Laura ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Bellora, Camille;  IBBL
Krishna, Abhimanyu;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Wampach, Linda ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Schneider, Jochen ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Hogan, Angela;  IBBL
de Beaufort, Carine ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Wilmes, Paul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
External co-authors :
yes
Language :
English
Title :
Integrated multi-omics of the human gut microbiome in a case study of familial type 1 diabetes.
Publication date :
2016
Journal title :
Nature Microbiology
ISSN :
2058-5276
Publisher :
Nature Publishing Group, United Kingdom
Volume :
2
Pages :
16180
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
FnR Project :
FNR4771725 - Biomarkers For Alzheimer'S Disease And Parkinson'S Disease, 2012 (01/11/2012-31/10/2015) - Paul Wilmes
Funders :
FNR - Fonds National de la Recherche [LU]
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