Article (Scientific journals)
A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Shah, Pranjul; Fritz, Joëlle; Glaab, Enrico et al.
2016In Nature Communications, 7, p. 11535
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Keywords :
microfluidics; microbiome; co-culture; gastrointestinal; model; microarray analysis; confocal microscopy; PCR
Abstract :
[en] Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
Disciplines :
Microbiology
Laboratory medicine & medical technology
Life sciences: Multidisciplinary, general & others
Biotechnology
Author, co-author :
Shah, Pranjul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Fritz, Joëlle ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Glaab, Enrico  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Desai, Mahesh S.
Greenhalgh, Kacy ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Frachet Bour, Audrey ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Niegowska, Magdalena
Estes, Matthew
Jäger, Christian  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Seguin-Devaux, Carole
Zenhausern, Frederic
Wilmes, Paul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
External co-authors :
yes
Language :
English
Title :
A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Publication date :
2016
Journal title :
Nature Communications
ISSN :
2041-1723
Publisher :
Nature Pub.lishing Group, London, United Kingdom
Volume :
7
Pages :
11535
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
FnR Project :
FNR
FNR11014639 - A Microfluidics-based Drug Discovery Platform Emulating The Human Microbiome On Chip, 2015 (01/01/2016-30/04/2017) - Paul Wilmes
Name of the research project :
R-STR-4014-00 > Eco-Systems Biology (Wilmes) > 01/01/2011 - 19/01/2048 > WILMES Paul
Funders :
Fonds National de la Recherche Luxembourg - FNR
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