Article (Scientific journals)
Dynamic regulation of microRNA expression following interferonγ- induced gene transcription
Reinsbach, Susanne; Nazarov, Petr V.; Philippidou, Demetra et al.
2012In RNA Biology, 9 (7), p. 987-989
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Keywords :
Dynamic expression; MicroRNA; Time-series microarrays; Transcriptional regulation
Abstract :
[en] MicroRNAs are major players in post-transcriptional gene regulation. Even small changes in miRNA levels may have profound consequences for the expression levels of target genes. Hence, miRNAs themselves need to be tightly, albeit dynamically, regulated. Here, we investigated the dynamic behavior of miRNAs over a wide time range following stimulation of melanoma cells with interferonγ (IFNγ), which activates the transcription factor STAT1. By applying several bioinformatic and statistical software tools for visualization and identification of differentially expressed miRNAs derived from time-series microarray experiments, 8.9% of 1105 miRNAs appeared to be directly or indirectly regulated by STAT1. Focusing on distinct dynamic expression patterns, we found that the majority of robust miRNA expression changes occurred in the intermediate time range (24-48 h). Three miRNAs (miR-27a, miR-30a and miR-34a) had a delayed regulation occurring at 72 h while none showed significant expression changes at early time points between 30 min and 6 h. Expression patterns of individual miRNAs were altered gradually over time or abruptly increased or decreased between two time points. Furthermore, we observed coordinated dynamic transcription of most miRNA clusters while few were found to be regulated independently of their genetic cluster. Most interestingly, several "star" or passenger strand sequences were specifically regulated over time while their "guide" strands were not. © 2012 Landes Bioscience.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Reinsbach, Susanne ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Nazarov, Petr V.;  Microarray Center, Centre de Recherche Public de la Santé, Luxembourg, Luxembourg
Philippidou, Demetra ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Schmitt, Martina;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit > Signal Transduction Laboratory
Wienecke, Anke ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Muller, Arnaud;  Centre de Recherche Public de la Santé Luxembourg > Microarray Center
Vallar, Laurent;  Centre de Recherche Public de la Santé Luxembourg > Microarray Center
Behrmann, Iris ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Kreis, Stephanie ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
External co-authors :
no
Language :
English
Title :
Dynamic regulation of microRNA expression following interferonγ- induced gene transcription
Publication date :
2012
Journal title :
RNA Biology
ISSN :
1555-8584
Publisher :
Landes Bioscience, United States
Volume :
9
Issue :
7
Pages :
987-989
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
University of Luxembourg (F1R-LSC-PUL-09MIRN)
Fondation Cancer (Luxembourg)
Commentary :
cited By (since 1996)6 Scopus
Available on ORBilu :
since 05 March 2015

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