Article (Scientific journals)
Interleukin-27 displays interferon-gamma-like functions in human hepatoma cells and hepatocytes.
Bender, Herdis; Wiesinger, Monique; Nordhoff, Carolin et al.
2009In Hepatology, 50 (2), p. 585-91
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Keywords :
Animals; Antimicrobial Cationic Peptides/metabolism; Carcinoma, Hepatocellular/metabolism; Cell Line, Tumor; Fibrinogen/metabolism; Gene Expression Regulation; Hepatocytes/metabolism; Humans; Interferon-gamma/metabolism; Interleukins/immunology/metabolism; Liver Neoplasms/metabolism; Male; Rats; Rats, Sprague-Dawley; STAT1 Transcription Factor/metabolism; STAT3 Transcription Factor/metabolism; Virus Replication
Abstract :
[en] Interleukin-27 (IL-27) is a cytokine belonging to the IL-6/IL-12 cytokine family. It is secreted by antigen-presenting cells, strongly acts on T cells, and also stimulates innate immune cells. In most studies, the effects of IL-27 on T cells were investigated; however, not much is known about possible effects of IL-27 on other cell types. IL-27 signals via the common IL-6-type cytokine receptor chain gp130 and the IL-27-specific chain WSX-1. Given the importance of gp130 in regulating liver responses such as the acute phase response or liver regeneration, we investigated whether IL-27 could also have a function in liver cells. We find that IL-27 stimulates hepatoma cells and hepatocytes by inducing a sustained signal transducer and activator of transcription (STAT)1 and STAT3 activation. Whereas the STAT3 mediated responses to IL-27 (gamma-fibrinogen and hepcidin induction) are not detectable, we observe an interferon-gamma (IFN-gamma)-like STAT1 response leading to the induction of interferon-regulated proteins such as STAT1, STAT2, interferon response factor (IRF)-1, IRF-9, myxovirus resistance A and guanylate binding protein 2. CONCLUSION: Our study provides evidence for a function of IL-27 in hepatoma cells and hepatocytes and shows that IL-27 responses are not restricted to the classical immune cells. Our results suggest that IL-27 exerts IFN-like functions in liver cells and that it can contribute to the antiviral response in these cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bender, Herdis
Wiesinger, Monique ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Nordhoff, Carolin
Schoenherr, Caroline
Haan, Claude ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Ludwig, Stephan
Weiskirchen, Ralf
Kato, Nobuyuki
Heinrich, Peter C.
Haan, Serge ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
External co-authors :
yes
Language :
English
Title :
Interleukin-27 displays interferon-gamma-like functions in human hepatoma cells and hepatocytes.
Publication date :
2009
Journal title :
Hepatology
ISSN :
1527-3350
Publisher :
John Wiley & Sons, Hoboken, United States - New Jersey
Volume :
50
Issue :
2
Pages :
585-91
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 10 January 2014

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