Cancer; Health Sciences; Model Organisms; Organoids; Animals; Mice; Neoplasm Metastasis; Disease Models, Animal; Colonoscopy/methods; Organoids/transplantation; Organoids/pathology; Colon/pathology; Colorectal Neoplasms/pathology; Colon; Colonoscopy; Colorectal Neoplasms; Neuroscience (all); Biochemistry, Genetics and Molecular Biology (all); Immunology and Microbiology (all)
Abstract :
[en] Orthotopic mouse models of colorectal cancer (CRC) better recapitulate the physiological processes of tumor development and metastatic dissemination. Here, we provide a protocol for colonoscopy-guided transplantation of organoids into the murine colon. We describe the steps for preparing mouse organoids, equipment, and mice for injections, as well as performing colonoscopy-guided mucosal injections and providing subsequent care. This model can be used to investigate various experimental setups, including survival, metastatic potential, and the effects of treatments. For complete details on the use and execution of this protocol, please refer to Felchle et al.1.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
ATATRI, Sura ✱; University of Luxembourg > Faculty of Science, Technology and Medicine > Department of Health, Medicine and Life Sciences > Team Elisabeth LETELLIER
MEYERS, Marianne ✱; University of Luxembourg > Faculty of Science, Technology and Medicine > Department of Health, Medicine and Life Sciences > Team Elisabeth LETELLIER
KLEIN, Eliane ; University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Health, Medicine and Life Sciences (DHML)
Brunner, Valentina; Center for Translational Cancer Research (TranslaTUM), School of Medicine and Health, Technical University of Munich, 81675 Munich, Germany, Institute of Experimental Cancer Therapy, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, 81675 Munich, Germany
Tschurtschenthaler, Markus; Center for Translational Cancer Research (TranslaTUM), School of Medicine and Health, Technical University of Munich, 81675 Munich, Germany, Institute of Experimental Cancer Therapy, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, 81675 Munich, Germany, Division of Translational Cancer Research, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), 69120 Heidelberg, Germany. Electronic address: markus.tschurtschenthaler@tum.de
LETELLIER, Elisabeth ; University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Health, Medicine and Life Sciences (DHML)
✱ These authors have contributed equally to this work.
External co-authors :
yes
Language :
English
Title :
Protocol for sub-mucosal orthotopic injection of organoids into murine colon to study tumor growth and metastasis.
National Research Fund Fondation Cancer Fondation du Pélican de Mie et Pierre Hippert-Faber EU Framework Programme for Research and Innovation Marie Skłodowska-Curie Actions Deutsche Forschungsgemeinschaft Horizon 2020 Framework Programme
