Neurology; Neurology (clinical); Cellular and Molecular Neuroscience
Abstract :
[en] Ageing is the primary risk factor for Parkinson's disease, yet the intricate interplay between these processes remains ambiguous. This position paper, a collaborative output from the PD-AGE consortium, addresses the urgent need for standardising methods in in vitro modelling. A panel of international experts recommends human induced pluripotent stem cell (iPSC)-derived models, with chemically induced ageing methods, such as the SLO cocktail, as a robust system. Furthermore, the consortium highlights the value of direct and semi-direct reprogramming for retaining donor-specific ageing phenotypes. The paper also outlines a prioritised panel of measurable parameters, categorised into senescence, inflammaging, omics profiling, and mitochondrial dysfunction, providing a consistent framework to enhance research reproducibility, investigating the nexus of ageing and Parkinson's. In addition, we provide links to SOPs ( https://doi.org/10.5281/zenodo.15056603 ) [1] to measure the key measurable ageing parameters outlined in this review to facilitate consistency and reproducibility within the field.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Bury, Alexander G; Sheffield Institute for Translational Neuroscience (SITraN), School of Medicine and Population Health, University of Sheffield, Sheffield, UK
Olejnik, Alicja; Sheffield Institute for Translational Neuroscience (SITraN), School of Medicine and Population Health, University of Sheffield, Sheffield, UK
Tocco, Chiara; Faculty of Pharmacy, University of Montreal, Montreal, QC, Canada
Saurat, Nathalie; The Center for Stem Cell Biology, Sloan Kettering Institute for Cancer Research, New York, NY, USA ; Developmental Biology Program, Sloan Kettering Institute for Cancer Research, New York, NY, USA ; Australian Regenerative Medicine Institute, Monash University, Clayton, Australia
Stephen, Elezabeth; Sheffield Institute for Translational Neuroscience (SITraN), School of Medicine and Population Health, University of Sheffield, Sheffield, UK
Hockemeyer, Dirk; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA ; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA ; Chan Zuckerberg Biohub, San Francisco, CA, USA
SCHWAMBORN, Jens Christian ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Developmental and Cellular Biology
Studer, Lorenz; The Center for Stem Cell Biology, Sloan Kettering Institute for Cancer Research, New York, NY, USA ; Developmental Biology Program, Sloan Kettering Institute for Cancer Research, New York, NY, USA
Mastroberardino, Pier Giorgio; Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, Netherlands ; Department of Life, Health and Environmental Sciences, Università degli Studi dell'Aquila, L'Aquila, Italy
Bolognin, Silvia; MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands
Kunath, Tilo; Institute for Stem Cell Research, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK
Korolchuk, Viktor I; Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle, UK
Drouin-Ouellet, Janelle; Faculty of Pharmacy, University of Montreal, Montreal, QC, Canada
Mortiboys, Heather; Sheffield Institute for Translational Neuroscience (SITraN), School of Medicine and Population Health, University of Sheffield, Sheffield, UK. H.Mortiboys@sheffield.ac.uk
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