[en] Conventional understanding dictates that mammalian neural stem cells (NSCs) exist only in the central nervous system. Here, we report that peripheral NSCs (pNSCs) exist outside the central nervous system and can be isolated from mouse embryonic limb, postnatal lung, tail, dorsal root ganglia and adult lung tissues. Derived pNSCs are distinct from neural crest stem cells, express multiple NSC-specific markers and exhibit cell morphology, self-renewing and differentiation capacity, genome-wide transcriptional profile and epigenetic features similar to control brain NSCs. pNSCs are composed of Sox1+ cells originating from neuroepithelial cells. pNSCs in situ have similar molecular features to NSCs in the brain. Furthermore, many pNSCs that migrate out of the neural tube can differentiate into mature neurons and limited glial cells during embryonic and postnatal development. Our discovery of pNSCs provides previously unidentified insight into the mammalian nervous system development and presents an alternative potential strategy for neural regenerative therapy.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Han, Dong ; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany. dhan23@hku.hk ; Stem Cell & Regenerative Medicine Consortium, School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. dhan23@hku.hk
Xu, Wan; Stem Cell & Regenerative Medicine Consortium, School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Jeong, Hyun-Woo ; Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, Münster, Germany
Park, Hongryeol; Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, Münster, Germany
Weyer, Kathrin; Developmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Belvaux, Luxembourg
Tsytsyura, Yaroslav; Department of Cellular Biophysics, Institute for Medical Physics and Biophysics, University of Münster, Münster, Germany
Stehling, Martin; Flow Cytometry Unit, Max Planck Institute for Molecular Biomedicine, Münster, Germany
Wu, Guangming; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany ; Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China ; Division of Basic Research, Guangzhou National Laboratory, Guangzhou, China
Lan, Guocheng; Stem Cell & Regenerative Medicine Consortium, School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Kim, Kee-Pyo ; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany ; Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
Renner, Henrik; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany
Han, Dong Wook; NUOXINTE Biotechnology, Suzhou, China
Chen, Yicong; Stem Cell & Regenerative Medicine Consortium, School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Gerovska, Daniela; Group of Computational Biology and Systems Biomedicine, Biogipuzkoa Health Research Institute, San Sebastian, Spain
Araúzo-Bravo, Marcos J; Group of Computational Biology and Systems Biomedicine, Biogipuzkoa Health Research Institute, San Sebastian, Spain ; IKERBASQUE, Basque Foundation for Science, Bilbao, Spain
Klingauf, Jürgen ; Department of Cellular Biophysics, Institute for Medical Physics and Biophysics, University of Münster, Münster, Germany ; IZKF Münster and Cluster of Excellence EXC 1003, Cells in Motion (CiM), Münster, Germany
SCHWAMBORN, Jens Christian ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Developmental and Cellular Biology
Adams, Ralf H ; Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, Münster, Germany ; University of Münster, Medical Faculty, Münster, Germany
Liu, Pentao ; Stem Cell & Regenerative Medicine Consortium, School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. pliu88@hku.hk ; Center for Translational Stem Cell Biology, Hong Kong, China. pliu88@hku.hk
Schöler, Hans R ; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany. office-schoeler@mpi-muenster.mpg.de
Open access funding provided by Max Planck Society.We thank M. Haustein for tissue section preparation; K. M\u00FCller for single-cell RNA-seq experiments; L. L\u00FCken for FACS experiments; M. Sinn for microarray sample preparation; and V. Episkopou, R. Lovell-Badge, S. Malas, G. Lapathitis, S. Gao, J. Chen, T. Braun, J. Kim, RIKEN BioResource Research Center, Centre for Comparative Medicine Research of the University of Hong Kong for help with transgenic mice. Finally, we thank A. Malapetsas for proofreading. This work was funded by the Max Planck Society\u2019s White Paper-Project \u2018Animal testing in the Max Planck Society\u2019. This work was supported by Health@InnoHK, Innovation Technology Commission.
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