In Vivo Phagocytosis Analysis of Amyloid Beta.

Tejera, Darío; HENEKA, Michael

doi:10.1007/978-1-4939-9658-2_21

No full text

Article (Scientific journals)

In Vivo Phagocytosis Analysis of Amyloid Beta.

Tejera, Darío; HENEKA, Michael

2019 • In Methods in Molecular Biology, 2034, p. 287 - 292

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/10993/61692

DOI
10.1007/978-1-4939-9658-2_21

PubMed
31392693

Files (0)Send to Details Statistics Bibliography Similar publications

Files

Full Text

No document available.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Alzheimer’s disease; Amyloid; Flow cytometry; Microglia; Neurodegeneration; Phagocytosis; Amyloid beta-Peptides; Mapt protein, mouse; tau Proteins; Amyloid beta-Peptides/metabolism; Animals; Mice; Phosphorylation; tau Proteins/metabolism; Alzheimer Disease/metabolism; Alzheimer Disease/pathology; Flow Cytometry; Microglia/metabolism; Microglia/pathology; Molecular Biology; Genetics

Abstract :

[en] Alzheimer's disease (AD) pathology is characterized by the presence of extracellular amyloid beta (Aβ), tau hyperphosphorylation, and neuroinflammation. One striking feature in the disease is the clustering of microglia around Aβ plaques. These cells exhibit a highly and chronically activated phenotype, performing a variety of functions, phagocytosis being one of them. Since Aβ phagocytosis by microglia could represent a key aspect in the pathogenesis and progression of the disease, robust and comprehensive methods to evaluate this process are needed. Here, we describe a detailed flow cytometry-based protocol for the analysis of Aβ phagocytosis in vivo.

Disciplines :

Neurology

Author, co-author :

Tejera, Darío; Department of Neurodegenerative Diseases and Gerontopsychiatry, University of Bonn, Bonn, Germany ; Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Bonn, Germany

HENEKA, Michael ; Department of Neurodegenerative Diseases and Gerontopsychiatry, University of Bonn, Bonn, Germany. Michael.heneka@ukbonn.de ; Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Bonn, Germany. Michael.heneka@ukbonn.de

External co-authors :

yes

Language :

English

Title :

In Vivo Phagocytosis Analysis of Amyloid Beta.

Publication date :

2019

Journal title :

Methods in Molecular Biology

ISSN :

1064-3745

eISSN :

1940-6029

Publisher :

Humana Press Inc., United States

Volume :

2034

Pages :

287 - 292

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://link.springer.com/content/pdf/10.1007/978-1-4939-9658-2_21

Available on ORBilu :

since 22 July 2024

Statistics

Number of views

26 (1 by Unilu)

Number of downloads

0 (0 by Unilu)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Winblad B, Amouyel P, Andrieu S et al (2016) Defeating Alzheimer’s disease and other dementias: a priority for European science and society. Lancet Neurol 15:455–532
Sasaguri H, Nilsson P, Hashimoto S et al (2017) APP mouse models for Alzheimer’s disease preclinical studies. EMBO J 36:2473–2487
Baruch K, Deczkowska A, Rosenzweig N et al (2016) PD-1 immune checkpoint blockade reduces pathology and improves memory in mouse models of Alzheimer’s disease. Nat Med 22:135–137
Heneka MT, Carson MJ, Khoury JE et al (2015) Neuroinflammation in Alzheimer’s disease. Lancet Neurol 14:388–405
Heneka MT, Kummer MP, Stutz A et al (2013) NLRP3 is activated in Alzheimer’s disease and contributes to pathology in APP/PS1 mice. Nature 493:674–678
Guerreiro R, Wojtas A, Bras J et al (2013) TREM2 variants in Alzheimer’s disease. N Engl J Med 368:117–127
Hollingworth P, Harold D, Sims R et al (2011) Common variants at ABCA7, MS4A6A/MS4A4E, EPHA1, CD33 and CD2AP are associated with Alzheimer’s disease. Nat Genet 43:429–435
Datta M, Staszewski O, Raschi E et al (2018) Histone deacetylases 1 and 2 regulate microglia function during development, homeostasis, and neurodegeneration in a context-dependent manner. Immunity 48:514–529.e6
Keren-shaul H, Spinrad A, Weiner A et al (2017) Article a unique microglia type associated with restricting development of alzheimer ’ s disease article a unique microglia type associated with restricting development of alzheimer ’ s disease. Cell 169(7):1276–1290.e17
Davalos D, Grutzendler J, Yang G et al (2005) ATP mediates rapid microglial response to local brain injury in vivo. Nat Neurosci 8:752–758
Hickman SE, Kingery ND, Ohsumi TK et al (2013) The microglial sensome revealed by direct RNA sequencing. Nat Neurosci 16:1896–1905
Condello C, Yuan P, Schain A, Grutzendler J (2015) Microglia constitute a barrier that prevents neurotoxic protofibrillar abeta42 hot-spots around plaques around plaques. Nat Commun 6:6176
Lucin KM, O’Brien CE, Bieri G et al (2013) Microglial beclin 1 regulates retromer trafficking and phagocytosis and is impaired in Alzheimer’s disease. Neuron 79:873–886
Paolicelli RC, Bolasco G, Pagani F et al (2011) Synaptic pruning by microglia is necessary for normal brain development. Science 333:1456–1458
Czirr E, Castello NA, Mosher KI et al (2017) Microglial complement receptor 3 regulates brain Aβ levels through secreted proteolytic activity. J Exp Med 214(4):1081–1092
Bradshaw EM, Chibnik LB, Keenan BT et al (2013) CD33 Alzheimer’s disease locus: altered monocyte function and amyloid biology. Nat Neurosci 16:848–850