Ca(2+) signaling; In vivo; Microglia; Peripheral inflammation; Lipopolysaccharides; Brain; Humans; Signal Transduction; Inflammation; Ca2+ signaling; Immunology; Endocrine and Autonomic Systems; Behavioral Neuroscience
Abstract :
[en] Peripheral inflammation is known to trigger a mirror inflammatory response in the brain, involving brain's innate immune cells - microglia. However, the functional phenotypes, which these cells adopt in the course of peripheral inflammation, remain obscure. In vivo two-photon imaging of microglial Ca2+ signaling as well as process motility reveals two distinct functional states of cortical microglia during a lipopolysaccharide-induced peripheral inflammation: an early "sensor state" characterized by dramatically increased intracellular Ca2+ signaling but ramified morphology and a later "effector state" characterized by slow normalization of intracellular Ca2+ signaling but hypertrophic morphology, substantial IL-1β production in a subset of cells as well as increased velocity of directed process extension and loss of coordination between individual processes. Thus, lipopolysaccharide-induced microglial Ca2+ signaling might represent the central element connecting receptive and executive functions of microglia.
Disciplines :
Neurology
Author, co-author :
Riester, Karin; Institute of Physiology, Department of Neurophysiology, Eberhard Karls University Tübingen, Tübingen, Germany
Brawek, Bianca; Institute of Physiology, Department of Neurophysiology, Eberhard Karls University Tübingen, Tübingen, Germany
Savitska, Daria; Institute of Physiology, Department of Neurophysiology, Eberhard Karls University Tübingen, Tübingen, Germany
Fröhlich, Nicole; Institute of Physiology, Department of Neurophysiology, Eberhard Karls University Tübingen, Tübingen, Germany
Zirdum, Elizabeta; Institute of Physiology, Department of Neurophysiology, Eberhard Karls University Tübingen, Tübingen, Germany
Mojtahedi, Nima; Institute of Physiology, Department of Neurophysiology, Eberhard Karls University Tübingen, Tübingen, Germany
HENEKA, Michael ; Department of Neurodegenerative Disease and Geriatric Psychiatry, University of Bonn, Bonn, Germany, German Center for Neurodegenerative Diseases, Bonn, Germany
Garaschuk, Olga; Institute of Physiology, Department of Neurophysiology, Eberhard Karls University Tübingen, Tübingen, Germany. Electronic address: olga.garaschuk@uni-tuebingen.de
External co-authors :
yes
Language :
English
Title :
In vivo characterization of functional states of cortical microglia during peripheral inflammation.
We thank A. Weible, G. Heck, and K. Schöntag for technical assistance. This work was partially supported by VolkswagenStiftung Grant 90233 to O.G. Appendix A
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