Critical appraisal of the side population assay in stem cell and cancer stem cell  research.

[en] The "Side Population" (SP) discrimination assay is a flow cytometry method used to detect stem cells based on the dye efflux properties of ABC transporters. We discuss the SP assay and its applications in stem cell biology, with an emphasis on the technical challenges related to sample preparation, data acquisition, analysis, and interpretation. We highlight the value of multicolor phenotyping, the impact of DNA ploidy, and the importance of distinguishing graft versus host cells for an appropriate SP discrimination. To improve the consistency and reliability of data between laboratories, we propose a set of recommendations for SP assay data reporting.

Disciplines :

Oncology

Author, co-author :

GOLEBIEWSKA, Anna ; NorLux Neuro-Oncology Laboratory, Oncology Department, Centre de Recherche Public de la Santé, 84 Val Fleuri, Luxembourg.

Brons, Nicolaas H C

Bjerkvig, Rolf

NICLOU, Simone P. ; orLux Neuro-Oncology Laboratory, Oncology Department, Centre de Recherche Public de la Santé, 84 Val Fleuri, L-1526 Luxembourg, Luxembourg

External co-authors :

yes

Language :

English

Title :

Critical appraisal of the side population assay in stem cell and cancer stem cell research.

Publication date :

04 February 2011

Journal title :

Cell Stem Cell

ISSN :

1934-5909

eISSN :

1875-9777

Publisher :

Cell Press, United States - Massachusetts

Volume :

Issue :

Pages :

136-47

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBilu :

since 23 February 2024

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Scopus citations^®

293

Scopus citations^®
without self-citations

284

OpenCitations

278

OpenAlex citations

337

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