Slit2 is a repellent for retinal ganglion cell axons.

Glycoproteins; Intercellular Signaling Peptides and Proteins; Nerve Tissue Proteins; Recombinant Proteins; Semaphorin-3A; Nerve Growth Factor; R6FXH13RRC (Slit homolog 2 protein); Animals; Axons/drug effects/physiology; Brain/physiology; Cattle; Cell Membrane/physiology; Chick Embryo; Ganglia, Spinal/embryology; Glycoproteins/pharmacology; Humans; Mice; Nerve Fibers/physiology; Nerve Growth Factor/pharmacology; Nerve Tissue Proteins/genetics/pharmacology/physiology; Organ Culture Techniques; Recombinant Proteins/metabolism/pharmacology; Retinal Ganglion Cells/drug effects/physiology; Transcription, Genetic

Abstract :

[en] We set out to isolate inhibitory guidance cues that affect retinal ganglion cell (RGC) axons in vitro and that could potentially be involved in RGC pathfinding decisions. Here we describe the biochemical purification of an RGC growth cone collapsing factor from bovine brain membranes and its identification as Slit2. Recombinant human Slit2 collapses and repels RGC growth cones from all quadrants of the chick retina. In the developing mouse visual system, slit2 is expressed in the eye, in the optic stalk, and in the ventral diencephalon. Slit2 expression is strong in anterior ventral diencephalic structures but is absent from the ventral midline where the optic chiasm forms. The putative receptors for Slits, robo1 and robo2, are expressed in the inner retinal layer in which RGCs are located. A comparison of the expression patterns of Slit2 and retinal axon trajectories suggests that slit2 acts as a short range repellent for retinal ganglion cell axons.

Disciplines :

Oncology

Author, co-author :

NICLOU, Simone P. ; Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

Jia, L

Raper, J A

External co-authors :

yes

Language :

English

Title :

Slit2 is a repellent for retinal ganglion cell axons.

Publication date :

01 July 2000

Journal title :

Journal of Neuroscience

ISSN :

0270-6474

eISSN :

1529-2401

Publisher :

Society for Neuroscience, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

4962-74

Peer reviewed :

Peer Reviewed verified by ORBi

Funding number :

R01 NS026527/NS/NINDS NIH HHS/United States; R01-NS26527/NS/NINDS NIH HHS/United States; R01-NS33656/NS/NINDS NIH HHS/United States

Available on ORBilu :

since 19 February 2024

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Scopus citations^®

146

Scopus citations^®
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137

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130

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164

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