Lentiviral-mediated transfer of CNTF to schwann cells within reconstructed  peripheral nerve grafts enhances adult retinal ganglion cell survival and axonal regeneration.

Hu, Ying; Leaver, Simone G; Plant, Giles W; Hendriks, William T J; NICLOU, Simone P.; Verhaagen, Joost; Harvey, Alan R; Cui, Qi

doi:10.1016/j.ymthe.2005.01.016

Article (Scientific journals)

Lentiviral-mediated transfer of CNTF to schwann cells within reconstructed peripheral nerve grafts enhances adult retinal ganglion cell survival and axonal regeneration.

Hu, Ying; Leaver, Simone G; Plant, Giles W et al.

2005 • In Molecular Therapy, 11 (6), p. 906-15

Peer Reviewed verified by ORBi

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https://hdl.handle.net/10993/60220

DOI
10.1016/j.ymthe.2005.01.016

PubMed
15922961

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Keywords :

Ciliary Neurotrophic Factor; RNA, Messenger; Animals; Axons/physiology; Ciliary Neurotrophic Factor/analysis/genetics/metabolism; Genetic Vectors/genetics; Lentivirus/genetics; Myelin Sheath/metabolism; Peripheral Nerves/cytology/transplantation; RNA, Messenger/analysis/metabolism; Rats; Rats, Sprague-Dawley; Regeneration; Retinal Ganglion Cells/cytology/physiology; Schwann Cells/chemistry/metabolism/transplantation; Tissue Engineering/methods; Transduction, Genetic

Abstract :

[en] We recently described a method for reconstituting peripheral nerve (PN) sheaths using adult Schwann cells (SCs). Reconstructed PN tissue grafted onto the cut optic nerve supports the regeneration of injured adult rat retinal ganglion cell (RGC) axons. To determine whether genetic manipulation of such grafts can further enhance regeneration, adult SCs were transduced with lentiviral vectors encoding either ciliary neurotrophic factor (LV-CNTF) or green fluorescent protein (LV-GFP). SCs expressed transgenes for at least 4 weeks after transplantation. There were high levels of CNTF mRNA and CNTF protein in PN grafts containing LV-CNTF-transduced SCs. Mean RGC survival was significantly increased with these grafts (11,863/retina) compared with LV-GFP controls (7064/retina). LV-CNTF-transduced SCs enhanced axonal regeneration to an even greater extent (3097 vs 393 RGCs/retina in LV-GFP controls). Many regenerated axons were myelinated. The use of genetically modified, reconstituted PN grafts to bridge tissue defects may provide new therapeutic strategies for the treatment of both CNS and PNS injuries.

Disciplines :

Neurology

Author, co-author :

Hu, Ying; School of Anatomy and Human Biology, Western Australian Institute for Medical Research, UWA Centre for Medical Research, Perth, Australia.

Leaver, Simone G

Plant, Giles W

Hendriks, William T J

NICLOU, Simone P. ; Netherlands Institute for Brain Research, 1105 AZ Amsterdam, The Netherlands

Verhaagen, Joost

Harvey, Alan R

Cui, Qi

External co-authors :

yes

Language :

English

Title :

Lentiviral-mediated transfer of CNTF to schwann cells within reconstructed peripheral nerve grafts enhances adult retinal ganglion cell survival and axonal regeneration.

Publication date :

June 2005

Journal title :

Molecular Therapy

ISSN :

1525-0016

eISSN :

1525-0024

Publisher :

Nature Publishing Group, United Kingdom

Volume :

Issue :

Pages :

906-15

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBilu :

since 16 February 2024

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Scopus citations^®

124

Scopus citations^®
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OpenCitations

108

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124

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