A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiation.

[en] The C2C12 cell line represents a simple in vitro model for cell differentiation. Here, we present a flow-cytometry-based pipeline to quantitate C2C12 cell differentiation based on myosin heavy-chain marker expression. We describe steps for cell seeding, transfection, drug treatment, differentiation, and labeling. We then detail procedures for flow cytometry acquisition and introduce the R script FlowFate for automated analysis, including the study of dose-dependent effects of GFP-tagged genes on differentiation. For complete details on the use and execution of this protocol, please refer to Chippalkatti et al. (2023).1.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

PARISI, Bianca ; University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Life Sciences and Medicine (DLSM)

Sünnen, Maxime; Cancer Cell Biology and Drug Discovery Group, Department of Life Sciences and Medicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg

CHIPPALKATTI, Rohan ; University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Life Sciences and Medicine (DLSM)

ABANKWA, Daniel ; University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Life Sciences and Medicine (DLSM)

External co-authors :

Language :

English

Title :

A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiation.

Publication date :

10 October 2023

Journal title :

STAR Protocols

eISSN :

2666-1667

Publisher :

Cell Press, United States

Volume :

Issue :

Pages :

102637

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S2666166723006044?httpAccept=text/xml

Funders :

Fonds National de la Recherche Luxembourg

Funding text :

We are indebted to Aurélien Ginolhac for advice on programming in R and implementation of FlowFate in Shiny. This work was supported by a grant from the Luxembourg National Research Fund (FNR) C19/BM/13673303-PolaRAS2 to D.K.A.

Available on ORBilu :

since 31 December 2023

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Bibliography

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