[en] Lymphocytic choriomeningitis virus (LCMV) is a frequently used animal model to study immune responses against acute and chronic viral infections. LCMV is a non-cytopathic virus, but destruction of infected cells by cytotoxic T lymphocytes (CTLs) can lead to severe damage of tissues. Virus-specific T cell responses have to be balanced. A low virus load leads to a strong T cell response and subsequently to viral control. In contrast, a high viral titer is associated with T cell exhaustion and chronic viral infections. During an intermediate LCMV viral load CD8+ T cells can cause immunopathology, which can have detrimental outcomes. The LCMV infection model offers the opportunity to study virus-specific CD4+ and CD8+ T cell responses during chronic and acute infections by quantifying LCMV-specific T cells by tetramer staining and measuring cytokine production and viral titers in different organs.
Disciplines :
Immunology & infectious disease
Author, co-author :
Grusdat, Melanie; Experimental and Molecular Immunology, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg, Immunology & Genetics, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg
Dostert, Catherine; Experimental and Molecular Immunology, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg, Immunology & Genetics, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg
BRENNER, Dirk ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Immunology and Genetics
External co-authors :
no
Language :
English
Title :
Chapter 9 - Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers.
Publication date :
2023
Main work title :
Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers
The authors would like to thank the National Cytometry Platform, the Luxembourg Institute of Health's Animal Welfare Structure and the National Institutes of Health (NIH) Tetramer Core Facility. The cell lines VL-4, MC-57 and the LCMV strains, that have been used to establish this protocol, were kindly provided by Philipp A. Lang (University of Düsseldorf, Germany). The authors are supported by the FNRS Televie program (7459719F).
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