Reference : Spaceflight-associated changes in immunoglobulin VH gene expression in the amphibian ...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/10993/5841

Title :	Spaceflight-associated changes in immunoglobulin VH gene expression in the amphibian Pleurodeles waltl
Language :	English
Author, co-author :	Bascove, Matthieu [Lorraine University, Vandpeuvre-lès-Nancy, France]
	Huin-Schohn, Cécile [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
	Gueguinou, Nathan [Lorraine University, Vandpeuvre-lès-Nancy, France]
	Tschirhart, Eric [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
	Frippiat, Jean-Pol [Lorraine University, Vandpeuvre-lès-Nancy, France]
Publication date :	2009
Journal title :	FASEB Journal
Publisher :	Federation of American Society for Experimental Biology
Volume :	23
Issue/season :	5
Pages :	1607-1615
Peer reviewed :	Yes (verified by ORBilu)
ISSN :	0892-6638
e-ISSN :	1530-6860
City :	Bethesda
Country :	MD
Abstract :	[en] Understanding why the immune system is depressed during spaceflight is of obvious importance for future human deep-space missions, such as the foreseen missions to Mars. However, little is known about the effects of these flights on humoral immunity. We previously immunized adult Pleurodeles waltl (urodele amphibian) onboard the Mir space station and showed that heavy-chain variable (VH) domains of specific IgM antibodies are encoded by genes belonging to the VHII and VHVI families. We have now determined how these animals use their individual VHII and VHVI genes by screening IgM heavy-chain cDNA libraries and by quantifying IgM heavy-chain transcripts encoded by these genes. Results were compared with those obtained using control animals immunized on Earth under the same conditions as onboard Mir. Our experiments revealed an increase in the expression of IgM heavy-chain mRNAs encoded by the VHII and VHVI.C genes and a strong decrease in the expression of IgM heavy-chain mRNAs encoded by the VHVI.A and VHVI.B genes in spaceflight animals. Consequently, different heavy-chain mRNAs are expressed by spaceflight animals, demonstrating that this environment affects the humoral response. These observations may be due to a change in B-cell selection under spaceflight conditions.
Permalink :	http://hdl.handle.net/10993/5841
DOI :	10.1096/fj.08-121327

	File(s) associated to this reference
	Fulltext file(s): File Commentary Version Size Access Limited access fasebj2009,23,1607-1615.pdf Author postprint 367.04 kB Request a copy

All documents in ORBi^lu are protected by a user license.

 

University of Luxembourg Library |  Feedback |  Legal notices Site Map