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Article (Scientific journals)
Endothelin-1 does not modulate O2.release and [Ca(2+)]i variations in resting or differentiated HL-60 cells
Gallois, A.; Bueb, Jean-Luc; Tschirhart, Eric
1996In Fundamental and Clinical Pharmacology, 10 (1), p. 28-32
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Keywords :
drug effects; Dose-Response Relationship, Drug; Endothelins; Fura-2; HL-60 Cells; Humans; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Superoxides; metabolism; pharmacology; Calcium
Abstract :
[en] Endothelin-1 (ET-1) by itself was not an effective stimulus for inducing superoxide (O2.) generation in human resting or DMSO-differentiated neutrophil-like HL-60 cells. ET-1 (0.01-100 nM) was not able to modulate O2. generation stimulated by the chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP, EC50 = 4.24 +/- 1.63 nM in the absence and 3.16 +/- 1.95 nM in the presence of ET-1). Neither did ET-1 (0.01-100 nM) promote the mobilization of intracellular calcium ions or modulate fMLP-induced [Ca(2+)]i increase in this model of human neutrophils. Phosphoramidon, a neutral endopeptidase inhibitor, was not able to reveal any biological (O2.) or biochemical ([Ca(2+)]i response to ET-1 in the absence or in the presence of fMLP in these cells. These results indicate that DMSO-differentiated neutrophil-like HL-60 cells are not sensitive to ET-1 in terms of O2. generation or [Ca(2+)]i variations.
Disciplines :
Biochemistry, biophysics & molecular biology
Identifiers :
UNILU:UL-ARTICLE-2008-213
Author, co-author :
Gallois, A.
Bueb, Jean-Luc ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Tschirhart, Eric ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Language :
English
Title :
Endothelin-1 does not modulate O2.release and [Ca(2+)]i variations in resting or differentiated HL-60 cells
Publication date :
1996
Journal title :
Fundamental and Clinical Pharmacology
ISSN :
1472-8206
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
10
Issue :
1
Pages :
28-32
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 09 September 2013

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