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Human umbilical cord blood-derived eosinophils cultured in the presence of IL-3 and IL-5 respond to fMLP with [Ca2+]i variation and O2- production
Zardini, D. M.; HEUSCHLING, Paul; Gallois, A. et al.
1997In Journal of Immunological Methods, 205 (1), p. 1-9
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Keywords :
pharmacology; Calcium; Cell Differentiation; Cells, Cultured; Eosinophils; Female; Fetal Blood; Humans; Interleukin-3; Interleukin-5; N-Formylmethionine Leucyl-Phenylalanine; Pregnancy; Reactive Oxygen Species; metabolism; cytology; Adult
Abstract :
[en] In the presence of interleukin-3 and interleukin-5, eosinophil precursors from human umbilical cord blood mononuclear cells were regularly differentiated into mature eosinophil-like cells expressing normal morphology and cyanide-resistant peroxidase. O2- production and [Ca2+]i rise were measured in these in vitro differentiated eosinophils after fMLP stimulation; with dihydrorhodamine-123 and fura-2, respectively. Umbilical cord blood-derived eosinophils responded to fMLP (0.01 nM to 3 microM) with a concentration-dependent production of O2- (EC50 = 63.1 +/- 17.2 nM; Emax = 71.0 +/- 6.2 pmol/min/10(6) cells). O2- production was correlated with an fMLP concentration-dependent increase in [Ca2+]i (EC50 = 32.5 +/- 14.9 nM; Emax = 200.0 +/- 23.9 nM). These results indicate that human umbilical cord blood-derived eosinophils demonstrate functional characteristics similar to adult human peripheral blood eosinophils after activation by fMLP. Therefore, the large numbers of eosinophils (2-3 x 10(6)/ml cord blood) which can be obtained by culture of human cord blood mononuclear cells may serve as a useful model for future studies which will provide insight into the pathogenesis of diseases associated with eosinophils.
Disciplines :
Biochemistry, biophysics & molecular biology
Identifiers :
UNILU:UL-ARTICLE-2008-212
Author, co-author :
Zardini, D. M.
HEUSCHLING, Paul ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC)
Gallois, A.
BUEB, Jean-Luc ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
TSCHIRHART, Eric ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Language :
English
Title :
Human umbilical cord blood-derived eosinophils cultured in the presence of IL-3 and IL-5 respond to fMLP with [Ca2+]i variation and O2- production
Publication date :
1997
Journal title :
Journal of Immunological Methods
ISSN :
0022-1759
Publisher :
Elsevier Science
Volume :
205
Issue :
1
Pages :
1-9
Peer reviewed :
Peer reviewed
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