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Pertussis toxin-sensitive G(i)-proteins and intracellular calcium sensitivity of vasoconstriction in the intact rat tail artery
Spitzbarth-Régrigny, E.; Petitcolin, M. A.; Bueb, Jean-Luc et al.
2000In British Journal of Pharmacology, 131 (7), p. 1337-44
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Keywords :
pharmacology; Pertussis Toxin; Norepinephrine; Membranes; Male; Immunoblotting; GTP-Binding Protein alpha Subunits, Gi-Go; Dose-Response Relationship, Drug; Calcium; Arteries; Potassium Chloride; Rats; blood supply; metabolism; physiology; drug effects; chemistry; Virulence Factors, Bordetella; Vasoconstriction; Tail; Rats, Wistar; Animals
Abstract :
[en] 1. We studied the involvement of pertussis toxin (PTX)-sensitive G-proteins in the sensitivity of arterial constriction to intracellular calcium ([Ca(2+)](i)) mobilization. 2. Vasoconstriction was measured in vitro in perfused, de-endothelialized rat tail arteries loaded with the calcium-sensitive dye, fura-2 and treated or not with PTX (30 - 1000 ng ml(-1)). Arteries were stimulated with noradrenaline (NA, 0.1 - 100 microM) or KCl (15 - 120 mM). 3. KCl elicited a smaller vasoconstrictor response (E(max)=94+/-8 mmHg) than NA (E(max)=198+/-9 mmHg) although [Ca(2+)](i) mobilization was similar (E(max)=123+/-8 and 135+/-7 nM for KCl and NA, respectively). PTX (1000 ng ml(-1)) had no effect on [Ca(2+)](i) mobilization but lowered NA- (but not KCl-) induced vasoconstriction (E(max)=118+/-7 mmHg). 4. G(i/o)-proteins were revealed by immunoblotting with anti-G(i alpha) and anti-G(o alpha) antibodies in membranes prepared from de-endothelialized tail arteries. [alpha(32)P]-ADP-ribosylation of G-proteins by PTX (1000 ng ml(-1)) was demonstrated in the intact rat tail artery (pixels in the absence of PTX: 3150, presence: 25053). 5. In conclusion, we suggest that smooth muscle cells possess a PTX-sensitive G(i)-protein-mediated intracellular pathway which amplifies [Ca(2+)](i) sensitivity of contraction in the presence of agonists such as NA.
Disciplines :
Biochemistry, biophysics & molecular biology
Identifiers :
UNILU:UL-ARTICLE-2008-537
Author, co-author :
Spitzbarth-Régrigny, E.
Petitcolin, M. A.
Bueb, Jean-Luc ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Tschirhart, Eric ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Atkinson, J.
Capdeville-Atkinson, C.
Language :
English
Title :
Pertussis toxin-sensitive G(i)-proteins and intracellular calcium sensitivity of vasoconstriction in the intact rat tail artery
Publication date :
2000
Journal title :
British Journal of Pharmacology
ISSN :
0007-1188
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
131
Issue :
7
Pages :
1337-44
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 09 September 2013

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