Article (Scientific journals)
Evolution of the murine gut resistome following broad-spectrum antibiotic treatment.
de Nies, Laura; Busi, Susheel Bhanu; Tsenkova, Mina et al.
2022In Nature Communications, 13 (1), p. 2296
Peer Reviewed verified by ORBi
 

Files


Full Text
de Nies et al, 2022.pdf
Publisher postprint (2.49 MB)
Download

All documents in ORBilu are protected by a user license.

Send to



Details



Keywords :
Animals; Anti-Bacterial Agents/pharmacology; Bacteria/genetics; Genes, Bacterial; Humans; Mammals/genetics; Metagenome; Mice; Plasmids
Abstract :
[en] The emergence and spread of antimicrobial resistance (AMR) represent an ever-growing healthcare challenge worldwide. Nevertheless, the mechanisms and timescales shaping this resistome remain elusive. Using an antibiotic cocktail administered to a murine model along with a longitudinal sampling strategy, we identify the mechanisms by which gut commensals acquire antimicrobial resistance genes (ARGs) after a single antibiotic course. While most of the resident bacterial populations are depleted due to the treatment, Akkermansia muciniphila and members of the Enterobacteriaceae, Enterococcaceae, and Lactobacillaceae families acquire resistance and remain recalcitrant. We identify specific genes conferring resistance against the antibiotics in the corresponding metagenome-assembled genomes (MAGs) and trace their origins within each genome. Here we show that, while mobile genetic elements (MGEs), including bacteriophages and plasmids, contribute to the spread of ARGs, integrons represent key factors mediating AMR in the antibiotic-treated mice. Our findings suggest that a single course of antibiotics alone may act as the selective sweep driving ARG acquisition and incidence in gut commensals over a single mammalian lifespan.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
de Nies, Laura ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology
Busi, Susheel Bhanu ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology
Tsenkova, Mina ;  University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Life Sciences and Medicine (DLSM)
Halder, Rashi ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Scientific Central Services
Letellier, Elisabeth ;  University of Luxembourg > Faculty of Science, Technology and Medicine (FSTM) > Department of Life Sciences and Medicine (DLSM)
Wilmes, Paul ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Systems Ecology
External co-authors :
no
Language :
English
Title :
Evolution of the murine gut resistome following broad-spectrum antibiotic treatment.
Publication date :
2022
Journal title :
Nature Communications
ISSN :
2041-1723
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
13
Issue :
1
Pages :
2296
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
Commentary :
© 2022. The Author(s).
Available on ORBilu :
since 09 March 2023

Statistics


Number of views
76 (7 by Unilu)
Number of downloads
16 (1 by Unilu)

Scopus citations®
 
11
Scopus citations®
without self-citations
10
OpenCitations
 
2
WoS citations
 
11

Bibliography


Similar publications



Contact ORBilu