Doctoral thesis (Dissertations and theses)
Population structure and phenotypical traits of Campylobacter jejuni circulating in Luxembourg
Nennig, Morgane


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Keywords :
C. jejuni; population structure; clonal expansion; persistence; metaphenotype
Abstract :
[en] Campylobacter is the leading cause of bacterial gastroenteritis worldwide. The most prevalent species, C. jejuni, is a strict microaerobic, capnophilic, and thermotolerant pathogen. Given its growth requirements, the ability of C. jejuni to persist in food environments and be transmitted throughout food processing has long puzzled scientists. This study aimed to compare the different genetic profiles of C. jejuni strains, isolated in Luxembourg, at the core genome (cg) and whole genome (wg) levels to elucidate its genetic population structure. Using phenotypical assays in controlled conditions and functional genomics analyses from wgMLST data, the study also investigated the possible link between phenotypic traits and emergence or persistence of genotypes. A high concordance in strain clustering was observed between genomic lineage classifications and the epidemic and endemic signals, regardless the three cgMLST typing schemes used. The higher genome stability within genomic lineages supports the hypothesis of a clonal expansion with monomorphic patterns over time and sources. A high correlation was observed between phenotypes and host-specific or generalist clonal complexes for oxidative stress, adhesion to inert surfaces, biofilm formation, and acclimation to aerobic conditions responses. These data allowed the establishment of metaphenotypes specific to the genomic lineages. Functional genomic analysis revealed factors that may contribute to the spatiotemporal survival of recurrent strains. These results also suggest the selection of better-adapted and persistent C. jejuni strains to environmental stresses throughout the transmission to human.
Research center :
Laboratoire National de Santé and INRAE
Disciplines :
Author, co-author :
Nennig, Morgane ;  University of Luxembourg > Faculty of Science, Technology and Medecine (FSTM) ; Laboratoire National de Santé > Microbiology > Epidemiology and Microbial Genomics Unit ; Institut Scientifique de Recherche Agronomique - INRA > UR-1194 Microbiologie et Chaine alimentaire
Language :
Title :
Population structure and phenotypical traits of Campylobacter jejuni circulating in Luxembourg
Alternative titles :
[fr] Structure des populations et traits phénotypiques de Campylobacter jejuni circulant au Luxembourg
Defense date :
14 March 2022
Number of pages :
xiv, 182 + 4
Institution :
Unilu - University of Luxembourg, Luxembourg
Oniris, Nantes, France
Degree :
Docteur de l'Université du Luxembourg en Biologie and Docteur d'Oniris en Microbiologie, Virologie et Parasitologie
Promotor :
President :
Jury member :
Meurens, François
Lehours, Philippe
Vandenberg, Olivier
FnR Project :
FNR11684203 - One Health Genomics Of Campylobacter Jejuni In Luxembourg, 2017 (15/03/2018-14/03/2022) - Catherine Ragimbeau
Name of the research project :
Funders :
FNR - Fonds National de la Recherche [LU]
RFI program "Food for Tomorrow"
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since 07 April 2022


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