Reference : Promoter characterization and genomic organization of the gene encoding integrin-link...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1
Melchior, C. A [Laboratoire Franco-Luxembourgeois de Recherche Biomédicale (CNRS/CRP-Santé), University Center, 162A, avenue de la Faiencerie L-1511, Luxembourg]
Kreis, Stephanie mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Janji, B. B [> >]
Kieffer, N. A [> >]
Biochimica et Biophysica Acta-Gene Structure and Expression
Elsevier Science
Yes (verified by ORBilu)
[en] Genomic sequence; Integrin; Integrin-linked kinase; Promoter activity
[en] Integrin-linked kinase (ILK)-1 is a 59-kDa serine-threonine protein kinase, which associates with the cytoplasmic domain of β1, β2 and β3 integrins and acts as a receptor proximal kinase regulating integrin-mediated signal transduction. We have recently identified an isoform of ILK (ILK-2), which is expressed, in a TGF-β1-dependent manner, in a highly invasive tumor cell line but not in normal adult tissues. In contrast, ILK-1 is ubiquitously expressed in normal tissues and is up-regulated in various tumors independent of TGF-β1. Here, we report the structural organization and the promoter activity of the human ILK-1 gene, contained within a 8.8-kb genomic fragment cloned from a human BAC library. The mature protein is encoded by 13 exons. The last coding exon contains the entire 3′ UTR of the ILK-1 gene, which overlaps with the complementary 3′ UTR sequence of the TAF2H gene, a TATA box binding protein-associated factor. A major transcriptional initiation start site was found 138 bp upstream of exon 1 in close proximity to a consensus initiator element (Inr). The ILK gene is transcribed by a TATA-less and CAAT-less promoter with typical features of housekeeping genes. The promoter activity was characterized by a luciferase reporter assay and the minimal sequence conferring promoter activity was 349 bp in size and located immediately upstream of exon 1. © 2002 Elsevier Science B.V. All rights reserved.
cited By (since 1996)11

There is no file associated with this reference.

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.