| Reference : Promoter characterization and genomic organization of the gene encoding integrin-link... |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/10993/4925 | |||
| Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1 | |
| English | |
| Melchior, C. A [Laboratoire Franco-Luxembourgeois de Recherche Biomédicale (CNRS/CRP-Santé), University Center, 162A, avenue de la Faiencerie L-1511, Luxembourg] | |
Kreis, Stephanie  [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| Janji, B. B [> >] | |
| Kieffer, N. A [> >] | |
| 2002 | |
| Biochimica et Biophysica Acta-Gene Structure and Expression | |
| Elsevier Science | |
| 1575 | |
| 1-3 | |
| 117-122 | |
| Yes (verified by ORBilu) | |
| 0167-4781 | |
| Amsterdam | |
| [en] Genomic sequence; Integrin; Integrin-linked kinase; Promoter activity | |
| [en] Integrin-linked kinase (ILK)-1 is a 59-kDa serine-threonine protein kinase, which associates with the cytoplasmic domain of β1, β2 and β3 integrins and acts as a receptor proximal kinase regulating integrin-mediated signal transduction. We have recently identified an isoform of ILK (ILK-2), which is expressed, in a TGF-β1-dependent manner, in a highly invasive tumor cell line but not in normal adult tissues. In contrast, ILK-1 is ubiquitously expressed in normal tissues and is up-regulated in various tumors independent of TGF-β1. Here, we report the structural organization and the promoter activity of the human ILK-1 gene, contained within a 8.8-kb genomic fragment cloned from a human BAC library. The mature protein is encoded by 13 exons. The last coding exon contains the entire 3′ UTR of the ILK-1 gene, which overlaps with the complementary 3′ UTR sequence of the TAF2H gene, a TATA box binding protein-associated factor. A major transcriptional initiation start site was found 138 bp upstream of exon 1 in close proximity to a consensus initiator element (Inr). The ILK gene is transcribed by a TATA-less and CAAT-less promoter with typical features of housekeeping genes. The promoter activity was characterized by a luciferase reporter assay and the minimal sequence conferring promoter activity was 349 bp in size and located immediately upstream of exon 1. © 2002 Elsevier Science B.V. All rights reserved. | |
| http://hdl.handle.net/10993/4925 | |
| 10.1016/S0167-4781(02)00247-6 | |
| http://www.scopus.com/inward/record.url?eid=2-s2.0-0037012729&partnerID=40&md5=280e4ee59a89c12455190d8ffc2444ab | |
| cited By (since 1996)11
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