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Article (Scientific journals)
Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1
Melchior, C. A; KREIS, Stephanie; Janji, B. B et al.
2002In Biochimica et Biophysica Acta-Gene Structure and Expression, 1575 (1-3), p. 117-122
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Keywords :
Genomic sequence; Integrin; Integrin-linked kinase; Promoter activity
Abstract :
[en] Integrin-linked kinase (ILK)-1 is a 59-kDa serine-threonine protein kinase, which associates with the cytoplasmic domain of β1, β2 and β3 integrins and acts as a receptor proximal kinase regulating integrin-mediated signal transduction. We have recently identified an isoform of ILK (ILK-2), which is expressed, in a TGF-β1-dependent manner, in a highly invasive tumor cell line but not in normal adult tissues. In contrast, ILK-1 is ubiquitously expressed in normal tissues and is up-regulated in various tumors independent of TGF-β1. Here, we report the structural organization and the promoter activity of the human ILK-1 gene, contained within a 8.8-kb genomic fragment cloned from a human BAC library. The mature protein is encoded by 13 exons. The last coding exon contains the entire 3′ UTR of the ILK-1 gene, which overlaps with the complementary 3′ UTR sequence of the TAF2H gene, a TATA box binding protein-associated factor. A major transcriptional initiation start site was found 138 bp upstream of exon 1 in close proximity to a consensus initiator element (Inr). The ILK gene is transcribed by a TATA-less and CAAT-less promoter with typical features of housekeeping genes. The promoter activity was characterized by a luciferase reporter assay and the minimal sequence conferring promoter activity was 349 bp in size and located immediately upstream of exon 1. © 2002 Elsevier Science B.V. All rights reserved.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Melchior, C. A;  Laboratoire Franco-Luxembourgeois de Recherche Biomédicale (CNRS/CRP-Santé), University Center, 162A, avenue de la Faiencerie L-1511, Luxembourg
KREIS, Stephanie ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Janji, B. B
Kieffer, N. A
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Title :
Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1
Publication date :
Journal title :
Biochimica et Biophysica Acta-Gene Structure and Expression
Publisher :
Elsevier Science, Amsterdam, Unknown/unspecified
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Issue :
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Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
cited By (since 1996)11 Scopus
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