Article (Scientific journals)
Approaches for completing metabolic networks through metabolite damage and repair discovery
Griffith, Corey; Walvekar, Adhish; Linster, Carole
2021In Current Opinion in Systems Biology, 28
Peer reviewed
 

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Abstract :
[en] Metabolites are prone to damage, either via enzymatic side reactions, which collectively form the underground metabolism, or via spontaneous chemical reactions. The resulting non-canonical metabolites that can be toxic, are mended by dedicated “metabolite repair enzymes.” Deficiencies in the latter can cause severe disease in humans, whereas inclusion of repair enzymes in metabolically engineered systems can improve the production yield of value-added chemicals. The metabolite damage and repair loops are typically not yet included in metabolic reconstructions and it is likely that many remain to be discovered. Here, we review strategies and associated challenges for unveiling non-canonical metabolites and metabolite repair enzymes, including systematic approaches based on high-resolution mass spectrometry, metabolome-wide side-activity prediction, as well as high-throughput substrate and phenotypic screens.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Griffith, Corey ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Enzymology and Metabolism
Walvekar, Adhish ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Enzymology and Metabolism
Linster, Carole  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Enzymology and Metabolism
External co-authors :
no
Language :
English
Title :
Approaches for completing metabolic networks through metabolite damage and repair discovery
Publication date :
09 September 2021
Journal title :
Current Opinion in Systems Biology
ISSN :
2452-3100
Publisher :
Elsevier
Volume :
28
Peer reviewed :
Peer reviewed
FnR Project :
FNR12661133 - Cellular Models Of A New Paediatric Neurodegenerative Disorder To Investigate Disease Mechanism, Develop Diagnostic Tools And Test Small Molecule Treatments, 2018 (01/05/2019-30/04/2022) - Carole Linster
Funders :
FNR - Fonds National de la Recherche [LU]
CE - Commission Européenne [BE]
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