Article (Scientific journals)
Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts.
Miroshnichenko, Svetlana; Timofeeva, Valeriia; Permykova, Elizaveta et al.
2019In Nanomaterials, 9 (4)
Peer Reviewed verified by ORBi
 

Files


Full Text
nanomaterials-09-00637.pdf
Publisher postprint (4.12 MB)
Download
Annexes
nanomaterials-09-00637-s001.pdf
(624.8 kB)
Download

All documents in ORBilu are protected by a user license.

Send to



Details



Keywords :
COOH plasma; cell adhesion and spreading; cell viability; freeze–thawed platelet-rich plasma immobilization; nanofibers; polycaprolactone
Abstract :
[en] Biodegradable nanofibers are extensively employed in different areas of biology and medicine, particularly in tissue engineering. The electrospun polycaprolactone (PCL) nanofibers are attracting growing interest due to their good mechanical properties and a low-cost structure similar to the extracellular matrix. However, the unmodified PCL nanofibers exhibit an inert surface, hindering cell adhesion and negatively affecting their further fate. The employment of PCL nanofibrous scaffolds for wound healing requires a certain modification of the PCL surface. In this work, the morphology of PCL nanofibers is optimized by the careful tuning of electrospinning parameters. It is shown that the modification of the PCL nanofibers with the COOH plasma polymers and the subsequent binding of NH(2) groups of protein molecules is a rather simple and technologically accessible procedure allowing the adhesion, early spreading, and growth of human fibroblasts to be boosted. The behavior of fibroblasts on the modified PCL surface was found to be very different when compared to the previously studied cultivation of mesenchymal stem cells on the PCL nanofibrous meshes. It is demonstrated by X-ray photoelectron spectroscopy (XPS) that the freeze-thawed platelet-rich plasma (PRP) immobilization can be performed via covalent and non-covalent bonding and that it does not affect biological activity. The covalently bound components of PRP considerably reduce the fibroblast apoptosis and increase the cell proliferation in comparison to the unmodified PCL nanofibers or the PCL nanofibers with non-covalent bonding of PRP. The reported research findings reveal the potential of PCL matrices for application in tissue engineering, while the plasma modification with COOH groups and their subsequent covalent binding with proteins expand this potential even further. The use of such matrices with covalently immobilized PRP for wound healing leads to prolonged biological activity of the immobilized molecules and protects these biomolecules from the aggressive media of the wound.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Miroshnichenko, Svetlana
Timofeeva, Valeriia
Permykova, Elizaveta
Ershov, Sergey ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Physics and Materials Science Research Unit
Kiryukhantsev-Korneev, Philip
Dvořaková, Eva
Shtansky, Dmitry V.
Zajíčková, Lenka
Solovieva, Anastasiya
Manakhov, Anton
External co-authors :
yes
Language :
English
Title :
Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts.
Publication date :
April 2019
Journal title :
Nanomaterials
ISSN :
2079-4991
Publisher :
MDPI AG, Basel, Switzerland
Volume :
9
Issue :
4
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
Available on ORBilu :
since 14 May 2020

Statistics


Number of views
68 (1 by Unilu)
Number of downloads
42 (0 by Unilu)

Scopus citations®
 
45
Scopus citations®
without self-citations
37
OpenCitations
 
38
WoS citations
 
43

Bibliography


Similar publications



Contact ORBilu