Article (Scientific journals)
Application of SWATH Proteomics to Mouse Biology.
Wu, Yibo; Williams, Evan; Aebersold, Ruedi
2017In Current Protocols in Mouse Biology, 7 (2), p. 130-143
Peer reviewed
 

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Keywords :
Animals; Mass Spectrometry; Mice; Proteome; Proteomics; Reproducibility of Results; systems biology
Abstract :
[en] The quantitative measurement of the proteome has been shown to yield new insights into physiology and cell biology that cannot be determined from the genome and transcriptome because the quantitative relationship between transcriptome and proteome is complex. MS-based proteomics techniques, such as SWATH-MS, have recently advanced to the point at which they may be reliably applied by biologists who are not specialists in mass spectrometry. Here we provide standard protocols for preparation of tissue samples for input into the SWATH-MS analytical pipeline. These protocols are designed for high-throughput processing of tissues with >/=5 mg of sample available for analysis. Studies with extremely limited amounts of tissue should consider PCT-SWATH. An experienced single user should be able to process 48 samples per day for injection into the mass spectrometer, or up to 144 samples a week. The machine time necessary for running these samples with SWATH is approximately 1.5 hr per sample. Data acquisition protocols are also provided. (c) 2017 by John Wiley & Sons, Inc.
Disciplines :
Genetics & genetic processes
Author, co-author :
Wu, Yibo
Williams, Evan  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Aebersold, Ruedi
External co-authors :
yes
Language :
English
Title :
Application of SWATH Proteomics to Mouse Biology.
Publication date :
2017
Journal title :
Current Protocols in Mouse Biology
ISSN :
2161-2617
Publisher :
John Wiley and Sons
Volume :
7
Issue :
2
Pages :
130-143
Peer reviewed :
Peer reviewed
Commentary :
Copyright (c) 2017 John Wiley & Sons, Inc.
Available on ORBilu :
since 11 May 2020

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