Reference : Multi-omic measurements of heterogeneity in HeLa cells across laboratories.
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/10993/43142
Multi-omic measurements of heterogeneity in HeLa cells across laboratories.
English
Liu, Yansheng [> >]
Mi, Yang [> >]
Mueller, Torsten [> >]
Kreibich, Saskia [> >]
Williams, Evan mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Van Drogen, Audrey [> >]
Borel, Christelle [> >]
Frank, Max [> >]
Germain, Pierre-Luc [> >]
Bludau, Isabell [> >]
Mehnert, Martin [> >]
Seifert, Michael [> >]
Emmenlauer, Mario [> >]
Sorg, Isabel [> >]
Bezrukov, Fedor [> >]
Bena, Frederique Sloan [> >]
Zhou, Hu [> >]
Dehio, Christoph [> >]
Testa, Giuseppe [> >]
Saez-Rodriguez, Julio [> >]
Antonarakis, Stylianos E. [> >]
Hardt, Wolf-Dietrich [> >]
Aebersold, Ruedi [> >]
2019
Nature biotechnology
37
3
314-322
Yes (verified by ORBilu)
1087-0156
1546-1696
United States
[en] DNA Copy Number Variations/genetics ; Genome, Human/genetics ; Genomics/standards ; HeLa Cells ; Humans ; Proteome/genetics ; Reproducibility of Results ; Transcriptome/genetics
[en] Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14 stock HeLa samples from 13 international laboratories. We cultured cells in uniform conditions and profiled genome-wide copy numbers, mRNAs, proteins and protein turnover rates in each cell line. We discovered substantial heterogeneity between HeLa variants, especially between lines of the CCL2 and Kyoto varieties, and observed progressive divergence within a specific cell line over 50 successive passages. Genomic variability has a complex, nonlinear effect on transcriptome, proteome and protein turnover profiles, and proteotype patterns explain the varying phenotypic response of different cell lines to Salmonella infection. These findings have implications for the interpretation and reproducibility of research results obtained from human cultured cells.
http://hdl.handle.net/10993/43142
10.1038/s41587-019-0037-y

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Limited access
41587-019-0037-y FINAL.pdfPublisher postprint4.83 MBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.