Article (Scientific journals)
Metabolite profiling of the cold adaptation of Pseudomonas putida KT2440 and cold‐sensitive mutants
Dethlefsen, Sarah; Jäger, Christian; Klockgether, Jens et al.
2019In Environmental Microbiology Reports, p. 1758--2229.12793
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Abstract :
[en] Free-living bacteria such as Pseudomonas putida are frequently exposed to temperature shifts and non-optimal growth conditions. We compared the transcriptome and metabolome of the cold adaptation of Pseudomonas putida KT2440 and isogenic cold-sensitive transposon mutants carrying transposons in their cbrA, cbrB, pcnB, vacB and bipA genes. P. putida changes the mRNA expression of about 43% of all annotated ORFs during this initial phase of cold adaptation, but only a small number of six to 93 genes were differentially expressed at 10°C between wild type strain and the individual mutants. The spectrum of metabolites underwent major changes during cold adaptation particularly in the mutants. Both KT2440 strain and the mutants increased the levels of the most abundant sugars and amino acids which were more pronounced in the cold-sensitive mutants. All mutants depleted their pools for core metabolites of aromatic and sugar metabolism, but increased their pool of polar amino acids which should be advantageous to cope with the cold stress.
Disciplines :
Microbiology
Author, co-author :
Dethlefsen, Sarah
Jäger, Christian  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Klockgether, Jens
Schomburg, Dietmar
Tümmler, Burkhard
External co-authors :
yes
Language :
English
Title :
Metabolite profiling of the cold adaptation of Pseudomonas putida KT2440 and cold‐sensitive mutants
Publication date :
2019
Journal title :
Environmental Microbiology Reports
ISSN :
1758-2229
Publisher :
Wiley-Blackwell, United States
Pages :
1758--2229.12793
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBilu :
since 01 October 2019

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