Reference : Bioactive nanotopographies for the control of mesenchymal stem cell differentiation f...
Dissertations and theses : Doctoral thesis
Physical, chemical, mathematical & earth Sciences : Chemistry
Physical, chemical, mathematical & earth Sciences : Physics
Engineering, computing & technology : Materials science & engineering
Physics and Materials Science
Bioactive nanotopographies for the control of mesenchymal stem cell differentiation for applications in bone tissue engineering
Realista Coelho Dos Santos Pedrosa, Catarina mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > >]
University of Luxembourg, ​​Luxembourg
University of Bordeaux, ​Bordeaux, ​​France
Docteur en Physique
Krishnamoorthy, Sivashankar mailto
Durrieu, Marie-Christine mailto
Pêgo, Ana Paula mailto
Nielsen, Lars Pleth mailto
[en] Mesenchymal stem cells ; Biomaterials ; Material nanostructuration ; Block copolymer self-assembly ; Surface chemistry
[en] Nanotopography with length scales of the order of extracellular matrix elements offers the possibility of regulating cell behavior. Investigation of the impact of nanotopography on cell response has been limited by inability to precisely control geometries, especially at high spatial resolutions, and across practically large areas. This work allowed the fabrication of well-controlled and periodic nanopillar arrays of silicon to investigate their impact on osteogenic differentiation of human mesenchymal stem cells (hMSCs). Silicon nanopillar arrays with critical dimensions in the range of 40-200 nm, exhibiting standard deviations below 15% across full wafers were realized using self-assembly of block copolymer colloids. To investigate if modifications of surface chemistry could further improve the modulation of hMSC differentiation, mimetic peptides were grafted on the fabricated nanoarrays. A peptide known for its ability to ameliorate cell adhesion (RGD peptide), a synthetic peptide able to enhance osteogenesis (BMP-2 mimetic peptide), and a combination or both molecules were covalently grafted on the nanostructures. Immunofluorescence and quantitative polymerase chain reaction (RT-qPCR) measurements reveal clear dependence of osteogenic differentiation of hMSCs on the diameter and periodicity of the arrays. Moreover, the differentiation of hMSCs was found to be dependent on the age of the donor. Surface functionalization allowed additional enhancement of the expression of osteogenic markers, in particular when RGD peptide and BMP-2 mimetic peptide were co-immobilized. These findings can contribute for the development of personalized treatments of bone diseases, namely novel implant nanostructuring depending on patient age.
H2020 ; 641640 - EJD-FunMat - European Joint Doctorate in Functional Materials Research

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