Reference : The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Computational Sciences
The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling
Pfau, Thomas mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Christian, Nils [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Masakapalli, Shyam K. []
Sweetlove, Lee J. []
Poolman, Mark G. []
Ebenhöh, Oliver []
Scientific Reports
Nature Publishing Group
United Kingdom
[en] Plant Metabolism ; Constraint Based Modelling ; Medicago Truncatula ; Sinorhizobium Meliloti ; Symbiosis
[en] Genome-scale metabolic network models can be used for various analyses including the prediction of metabolic responses to changes in the environment. Legumes are well known for their rhizobial symbiosis that introduces nitrogen into the global nutrient cycle. Here, we describe a fully compartmentalised, mass and charge-balanced, genome-scale model of the clover Medicago truncatula, which has been adopted as a model organism for legumes. We employed flux balance analysis to demonstrate that the network is capable of producing biomass components in experimentally observed proportions, during day and night. By connecting the plant model to a model of its rhizobial symbiont, Sinorhizobium meliloti, we were able to investigate the effects of the symbiosis on metabolic fluxes and plant growth and could demonstrate how oxygen availability influences metabolic exchanges between plant and symbiont, thus elucidating potential benefits of inter organism amino acid cycling. We thus provide a modelling framework, in which the interlinked metabolism of plants and nodules can be studied from a theoretical perspective.
This article is licensed under a Creative Commons Attribution 4.0 International License,
FP7 ; 316427 - ACCLIPHOT - Environmental Acclimation of Photosynthesis

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