Reference : Immunoresponsive Gene 1 and Itaconate Inhibit Succinate Dehydrogenase to Modulate Int...
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
Immunoresponsive Gene 1 and Itaconate Inhibit Succinate Dehydrogenase to Modulate Intracellular Succinate Levels.
Cordes, Thekla [> >]
Wallace, Martina [> >]
Michelucci, Alessandro mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Divakaruni, Ajit S. [> >]
Sapcariu, Sean C. [> >]
Sousa, Carole mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > > ; Luxembourg Institute of Health - LIH]
Koseki, Haruhiko [> >]
Cabrales, Pedro [> >]
Murphy, Anne N. [> >]
Hiller, Karsten [> >]
Metallo, Christian M. [> >]
The Journal of biological chemistry
Yes (verified by ORBilu)
United States
[en] Animals ; Cell Line ; Hydro-Lyases/physiology ; Lipopolysaccharides/pharmacology ; Macrophages/drug effects/metabolism ; Mice ; Succinate Dehydrogenase/antagonists & inhibitors ; Succinates/pharmacology ; Succinic Acid/metabolism ; immunoresponsive gene 1 (Irg1) ; inflammation ; itaconate ; macrophage ; metabolic regulation ; mitochondrial metabolism ; mitochondrial respiratory chain complex ; succinate ; succinate dehydrogenase (SDH)
[en] Metabolic reprogramming is emerging as a hallmark of the innate immune response, and the dynamic control of metabolites such as succinate serves to facilitate the execution of inflammatory responses in macrophages and other immune cells. Immunoresponsive gene 1 (Irg1) expression is induced by inflammatory stimuli, and its enzyme product cis-aconitate decarboxylase catalyzes the production of itaconate from the tricarboxylic acid cycle. Here we identify an immunometabolic regulatory pathway that links Irg1 and itaconate production to the succinate accumulation that occurs in the context of innate immune responses. Itaconate levels and Irg1 expression correlate strongly with succinate during LPS exposure in macrophages and non-immune cells. We demonstrate that itaconate acts as an endogenous succinate dehydrogenase inhibitor to cause succinate accumulation. Loss of itaconate production in activated macrophages from Irg1(-/-) mice decreases the accumulation of succinate in response to LPS exposure. This metabolic network links the innate immune response and tricarboxylic acid metabolism to function of the electron transport chain.
(c) 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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