[en] BACKGROUND: Increasing evidence connects the gut microbiota and the onset and/or phenotype of Parkinson's disease (PD). Differences in the abundances of specific bacterial taxa have been reported in PD patients. It is, however, unknown whether these differences can be observed in individuals at high risk, for example, with idiopathic rapid eye movement sleep behavior disorder, a prodromal condition of alpha-synuclein aggregation disorders including PD. OBJECTIVES: To compare microbiota in carefully preserved nasal wash and stool samples of subjects with idiopathic rapid eye movement sleep behavior disorder, manifest PD, and healthy individuals. METHODS: Microbiota of flash-frozen stool and nasal wash samples from 76 PD patients, 21 idiopathic rapid eye movement sleep behavior disorder patients, and 78 healthy controls were assessed by 16S and 18S ribosomal RNA amplicon sequencing. Seventy variables, related to demographics, clinical parameters including nonmotor symptoms, and sample processing, were analyzed in relation to microbiome variability and controlled differential analyses were performed. RESULTS: Differentially abundant gut microbes, such as Akkermansia, were observed in PD, but no strong differences in nasal microbiota. Eighty percent of the differential gut microbes in PD versus healthy controls showed similar trends in idiopathic rapid eye movement sleep behavior disorder, for example, Anaerotruncus and several Bacteroides spp., and correlated with nonmotor symptoms. Metagenomic sequencing of select samples enabled the reconstruction of genomes of so far uncharacterized differentially abundant organisms. CONCLUSION: Our study reveals differential abundances of gut microbial taxa in PD and its prodrome idiopathic rapid eye movement sleep behavior disorder in comparison to the healthy controls, and highlights the potential of metagenomics to identify and characterize microbial taxa, which are enriched or depleted in PD and/or idiopathic rapid eye movement sleep behavior disorder. (c) 2017 International Parkinson and Movement Disorder Society.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group) ULHPC - University of Luxembourg: High Performance Computing
Life sciences: Multidisciplinary, general & others Microbiology
Author, co-author :
Heintz, Anna ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Pandey, Urvashi; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Ecosystems Biology
Oertel, Wolfgang H.
Wilmes, Paul ; University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
External co-authors :
The nasal and gut microbiome in Parkinson's disease and idiopathic rapid eye movement sleep behavior disorder.
Publication date :
Journal title :
John Wiley & Sons, Hoboken, United States - New York
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
FnR Project :
FNR10404093 - Non-invasive Microbiome-derived Multi-omic Biomarkers For Early-stage Colorectal Cancer Detection, 2015 (01/01/2016-30/04/2019) - Paul Wilmes
Name of the research project :
FNR11333923 > Paul Wilmes > MiBiPa > Non-invasive microbiome-derived multi-omic biomarkers for the early-stage detection and stratification of Parkinson’s disease > 01/09/2017 > 31/08/2020 > 2016
FNR - Fonds National de la Recherche [LU] Rotary Club Luxembourg MJFF - Michael J. Fox Foundation for Parkinson's Research [US-NY] Charitable Hertie Foundation
(c) 2017 International Parkinson and Movement Disorder Society.