Article (Scientific journals)
Metabolic profiling of body fluids and multivariate data analysis
Trezzi, Jean-Pierre; Jäger, Christian; Galozzi, Sara et al.
2017In MethodsX, 4 (1), p. 95-103
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Keywords :
Metabolomics; Metabolite extraction; Blood; Saliva; Cerebrospinal fluid; GC-MS; Data analysis; Body fluids
Abstract :
[en] Metabolome analyses of body fluids are challenging due pre-analytical variations, such as pre-processing delay and temperature, and constant dynamical changes of biochemical processes within the samples. Therefore, proper sample handling starting from the time of collection up to the analysis is crucial to obtain high quality samples and reproducible results. A metabolomics analysis is divided into 4 main steps: 1) Sample collection, 2) Metabolite extraction, 3) Data acquisition and 4) Data analysis. Here, we describe a protocol for gas chromatography coupled to mass spectrometry (GC–MS) based metabolic analysis for biological matrices, especially body fluids. This protocol can be applied on blood serum/plasma, saliva and cerebrospinal fluid (CSF) samples of humans and other vertebrates. It covers sample collection, sample pre-processing, metabolite extraction, GC–MS measurement and guidelines for the subsequent data analysis.
Research center :
Luxembourg Centre for Systems Biomedicine (LCSB)
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Trezzi, Jean-Pierre ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Jäger, Christian  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Galozzi, Sara;  Functional Proteomics, Medizinisches Proteom-Center, Ruhr-University Bochum, Germany
Barkovits, Katalin;  Functional Proteomics, Medizinisches Proteom-Center, Ruhr-University Bochum, Germany
Marcus, Katrin;  Functional Proteomics, Medizinisches Proteom-Center, Ruhr-University Bochum, Germany
Mollenhauer, Brit;  Goettingen University, Germany
Hiller, Karsten;  TU Braunschweig > BRICS
External co-authors :
yes
Language :
English
Title :
Metabolic profiling of body fluids and multivariate data analysis
Publication date :
20 February 2017
Journal title :
MethodsX
ISSN :
2215-0161
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
4
Issue :
1
Pages :
95-103
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
Funders :
FNR - Fonds National de la Recherche [LU]
Available on ORBilu :
since 30 March 2017

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