Reference : LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lact...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/10993/28866

Title :	LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels
Language :	English
Author, co-author :	Trezzi, Jean-Pierre [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Bulla, Alexandre [Hôpitaux Universitaires de Genève - HUG > Département de Médecine Génétique et de Laboratoire > Sérothèque centrale]
	Bellora, Camille [Integrated Biobank of Luxembourg]
	Rose, Michael [Jena University Hospital > Institute of clinical chemistry and laboratory diagnostics and Integrierte Biobank Jena (IBBJ)]
	Lescuyer, Pierre [Hôpitaux Universitaires de Genève - HUG > Département de Médecine Génétique et de Laboratoire > Sérothèque centrale]
	Kiehntopf, Michael [Jena University Hospital > Institute of clinical chemistry and laboratory diagnostics and Integrierte Biobank Jena (IBBJ)]
	Hiller, Karsten [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Betsou, Fay [Integrated Biobank of Luxembourg]
Publication date :	Jun-2016
Journal title :	Metabolomics : Official journal of the Metabolomic Society
Volume :	12
Issue/season :	96
Peer reviewed :	Yes
Audience :	International
ISSN :	1573-3882
e-ISSN :	1573-3890
Keywords :	[en] Plasma processing ; Biobank ; Quality control ; Pre-analytics ; GC-MS ; Metabolomics
Abstract :	[en] Introduction Metabolome analysis is complicated by the continuous dynamic changes of metabolites in vivo and ex vivo. One of the main challenges in metabolomics is the robustness and reproducibility of results, partially driven by pre-analytical variations. Objectives The objective of this study was to analyse the impact of pre-centrifugation time and temperature, and to determine a quality control marker in plasma samples. Methods Plasma metabolites were measured by gas chromatography-mass spectrometry (GC–MS) and analysed with the MetaboliteDetector software. The metabolites, which were the most labile to pre-analytical variations, were further measured by enzymatic assays. A score was calculated for their use as quality control markers. Results The pre-centrifugation temperature was shown to be critical in the stability of plasma samples and had a significant impact on metabolite concentration profiles. In contrast, pre-centrifugation delay had only a minor impact. Based on the results of this study, whole blood should be kept on wet ice and centrifuged within maximum 3 h as a prerequisite for preparing EDTA plasma samples fit for the purpose of metabolome analysis. Conclusions We have established a novel blood sample quality control marker, the LacaScore, based on the ascorbic acid to lactic acid ratio in plasma, which can be used as an indicator of the blood pre-centrifugation conditions, and hence the suitability of the sample for metabolome analyses. This method can be applied in research institutes and biobanks, enabling assessment of the quality of their plasma sample collections.
Permalink :	http://hdl.handle.net/10993/28866
DOI :	10.1007/s11306-016-1038-1

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